Bovine CXCL9 (MIG) Recombinant Protein

Catalog Number:: RP0025B
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Bison bison bison (American buffalo), Bos grunniens (domestic yak), Bos indicus (zebu), Bos mutus (wild yak), Bos taurus (cattle)

Bovine CXCL9 (alias MIG, monokine induced by gamma interferon) (catalog RP0025B) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~11.9 kDa, 104 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across several bovine species: cattle, American buffalo, domestic yak, zebu, and wild yak. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used in immunology and cell-biology research to study chemokine signaling and leukocyte chemoattraction/migration (particularly CXCR3⁺ T cells and NK cells), inflammation and immune-cell trafficking; typical experimental uses include chemotaxis and cell-migration assays, receptor-binding and signaling studies, cell-culture stimulation and dosing, ELISA and neutralization assays, flow-cytometry controls, and antibody generation/validation.

Amino Acid Sequence	VPAIRNGRCS CINTSQGMIH PKSLKDLKQF APSPSCEKTE IIATMKNGNE ACLNPDLPEV KELIKEWEKQ VNQKKKQRKG KKYKKTKKVP KVKRSQRPSQ KKTT (104)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	11.9kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Bovine CXCL9 (C-X-C motif chemokine ligand 9), also known as MIG (monokine induced by interferon-γ), is a proinflammatory chemokine belonging to the CXC chemokine family, which also includes CXCL10 (IP-10) and CXCL11 (I-TAC) that regulate T-cell recruitment during interferon-driven immune responses. In cattle (Bos taurus), CXCL9 is produced by macrophages, dendritic cells, endothelial cells, and epithelial cells in response to interferon-γ (IFN-γ) and other inflammatory stimuli during infection or immune activation. CXCL9 signals primarily through the chemokine receptor CXCR3, expressed on activated T lymphocytes, natural killer (NK) cells, and other immune cells, promoting chemotaxis and accumulation of these cells at sites of infection or inflammation. In bovine health, CXCL9 contributes to cell-mediated immune responses against intracellular pathogens, including those responsible for diseases such as bovine tuberculosis caused by Mycobacterium bovis, where CXCL9 helps recruit Th1-type T cells and NK cells to infected tissues. Because of its role in interferon-regulated immune responses and T-cell trafficking, bovine CXCL9 is studied in veterinary immunology, infectious disease research, and vaccine development, particularly in investigations of host immune responses to mycobacterial infections and other intracellular pathogens in cattle.

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