Bovine IL-2 Recombinant Protein

Catalog Number:: RP0026B
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Bison bison bison (American buffalo), Bos gaurus (gaur), Bos indicus (zebu), Bos javanicus (banteng), Bos mutus (wild yak), Bos taurus (cattle), Bubalus bubalis (water buffalo)

Bovine IL-2 (catalog RP0026B) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~15.5 kDa, 135 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across several bovine species: cattle, American buffalo, gaur, zebu, banteng, wild yak, and water buffalo. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used in immunology and cell-biology research to study T-cell proliferation and activation, NK-cell function and cytokine signaling; typical experimental uses include cell-culture stimulation and dosing studies, ELISA and neutralization assays, flow-cytometry controls, and antibody generation/validation.

Amino Acid Sequence	APTSSSTGNT MKEVKSLLLD LQLLLEKVKN PENLKLSRMH TFDFYVPKVN ATELKHLKCL LEELKLLEEV LNLAPSKNLN PREIKDSMDN IKRIVLELQG SETRFTCEYD DATVNAVEFL NKWITFCQSI YSTMT (135)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	15.5 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Bovine IL-2 (Interleukin-2) is a central T cell-derived cytokine that regulates lymphocyte proliferation, differentiation, and immune homeostasis in cattle (Bos taurus). IL-2 is produced primarily by activated CD4⁺ and CD8⁺ T lymphocytes following antigen recognition and costimulatory signaling, and it functions through the IL-2 receptor complex (IL-2Rα/CD25, IL-2Rβ, and the common γ chain) to promote clonal expansion of antigen-specific T cells, enhance cytotoxic T lymphocyte and natural killer (NK) cell activity, and support development and maintenance of regulatory T cells (Tregs). In healthy cattle, basal IL-2 expression is generally low but increases during infection, vaccination, and immune stimulation. Altered IL-2 responses have been associated with bovine viral diarrhea virus (BVDV), bovine leukemia virus (BLV), Mycobacterium bovis (bovine tuberculosis), mastitis, and bovine respiratory disease complex. Cattle serve as important natural models for human tuberculosis, retroviral infection, and mucosal immune responses, as well as for vaccine development and immunomodulatory therapies. In veterinary and translational research, bovine IL-2 is widely used as a biomarker of T cell activation and cell-mediated immunity and is studied in the context of host-pathogen interactions, vaccine efficacy, and immune regulation in livestock health.

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