Bovine CCL2 (MCP-1) Recombinant Protein

Catalog Number:: RP0027B
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Bison bison bison (American buffalo), Bos indicus (zebu), Bos mutus (wild yak), Bos taurus (cattle)

Bovine CCL2 (catalog RP0027B) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile phosphate-buffered saline containing at least 0.1% carrier protein. The protein is ~8.8 kDa, 76 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across several bovine species: cattle, American buffalo, wild yak, and zebu. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used to study chemokine signaling and monocyte/macrophage chemoattraction and migration; typical experimental uses include chemotaxis and cell-migration assays, cell-culture stimulation and dosing studies, ELISA and neutralization assays, receptor-binding studies, flow-cytometry controls, Western blot controls, and antibody generation/validation.

Amino Acid Sequence	QPDAINSQVA CCYTFNSKKI SMQRLMNYRR VTSSKCPKEA VIFKTILGKE LCADPKQKWV QDSINYLNKK NQTPKP (76)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	8.8 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Bovine CCL2 (C-C motif chemokine ligand 2, also known as monocyte chemoattractant protein-1, MCP-1) is a pro-inflammatory chemokine that plays a central role in recruitment and activation of monocytes, macrophages, and memory T cells during immune responses in cattle (Bos taurus). CCL2 signals primarily through the CCR2 receptor, triggering intracellular signaling pathways that promote leukocyte migration, cytokine production, and coordination of innate and adaptive immune responses at sites of infection or tissue injury. In bovine health, CCL2 is particularly important in inflammatory conditions such as mastitis, where it mediates recruitment of monocytes and macrophages to infected mammary tissue, as well as in respiratory diseases associated with the bovine respiratory disease complex (BRDC) and infections such as Mycobacterium bovis. While CCL2-driven immune cell recruitment supports pathogen clearance and tissue repair, excessive or prolonged expression can contribute to tissue damage and chronic inflammation. As a biomarker of monocyte/macrophage recruitment and inflammatory activation, bovine CCL2 is widely studied in investigations of host-pathogen interactions, immune responses to bacterial and viral infections, and vaccine efficacy. Characterization of bovine CCL2 supports veterinary research aimed at improving disease resistance, understanding inflammatory disease mechanisms, and enhancing health and productivity in cattle.

Choose a size

Please choose a size to see price

RELATED MEDIA