Equine CCL2 (MCP-1) Recombinant Protein

Catalog Number:: RP0054E
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Equus caballus (horse), Equus przewalskii (Przewalski's horse)

Product Description

Equine CCL2 (alias MCP-1, monocyte chemoattractant protein-1) (catalog RP0054E) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~8.5 kDa, 76 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across horse and Przewalski's horse. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used to study chemokine signaling and monocyte/macrophage chemoattraction and migration; typical experimental uses include chemotaxis and cell-migration assays, cell-culture stimulation and dosing studies, ELISA and neutralization assays, receptor-binding studies, flow-cytometry controls, and antibody generation/validation.

Specifications

Amino Acid Sequence	QPDAINSPVT CCYTFTGKKI SSQRLGSYKR VTSSKCPKEA VIFKTILAKE ICADPEQKWV QDAVKQLDKK AQTPKP (76)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	8.5 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Equine CCL2 (C-C motif chemokine ligand 2, also known as monocyte chemoattractant protein-1, MCP-1) is a pro-inflammatory chemokine that plays a key role in recruitment and activation of monocytes, macrophages, and memory T cells during immune responses in horses (Equus caballus). CCL2 signals primarily through the CCR2 receptor, activating intracellular pathways that promote leukocyte migration, cytokine production, and coordination of innate and adaptive immune responses at sites of infection, inflammation, or tissue injury. In equine health, CCL2 is particularly important in inflammatory conditions such as equine asthma (recurrent airway obstruction), osteoarthritis, laminitis, and infectious diseases, where monocyte and macrophage recruitment contributes to tissue inflammation and repair. CCL2-mediated signaling also plays a role in joint inflammation by promoting infiltration of immune cells into synovial tissues during degenerative joint disease. While CCL2-driven immune cell trafficking is essential for pathogen defense and wound healing, excessive or chronic expression can contribute to persistent inflammation and tissue damage. As a biomarker of monocyte/macrophage recruitment and inflammatory activation, equine CCL2 is widely studied in investigations of respiratory disease, musculoskeletal injury, and immune responses to infection. Characterization of equine CCL2 supports veterinary research into inflammatory disease mechanisms, host-pathogen interactions, and development of targeted therapies to improve equine health and performance.

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