Bovine TNF alpha Recombinant Protein

Catalog Number:: RP0055B
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Bison bison bison (American buffalo), Bos indicus (zebu), Bos javanicus (banteng), Bos mutus (wild yak), Bos taurus (cattle), Bubalus bubalis (water buffalo), Bubalus kerabau (carabao)

Product Description

Bovine TNF-α (alias TNF-alpha) (catalog RP0055B) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~17.4 kDa, 157 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across several bovine species: cattle, American buffalo, zebu, banteng, wild yak, water buffalo, and carabao. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used in immunology and cell-biology research to study TNF-α signaling, inflammation, apoptosis and cytokine biology; typical experimental uses include cell-culture stimulation and dosing studies, cytotoxicity and apoptosis assays, ELISA and neutralization assays, receptor-binding studies, flow-cytometry and Western blot controls, and antibody generation/validation.

Specifications

Amino Acid Sequence	LRSSSQASSN KPVAHVVADI NSPGQLRWWD SYANALMANG VKLEDNQLVV PADGLYLIYS QVLFRGQGCP STPLFLTHTI SRIAVSYQTK VNILSAIKSP CHRETPEWAE AKPWYEPIYQ GGVFQLEKGD RLSAEINLPD YLDYAESGQV YFGIIAL (157)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	17.4 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Bovine Tumor Necrosis Factor Alpha (TNF-α) (also known as TNF or cachectin) is a proinflammatory cytokine that plays a central role in innate immune responses, inflammation, and host defense against pathogens in cattle (Bos taurus). TNF-α is a member of the tumor necrosis factor (TNF) superfamily, which includes related cytokines such as TNF-β (lymphotoxin-α), Fas ligand (FasL), CD40 ligand (CD40L), and TRAIL, molecules involved in regulating immune signaling, apoptosis, and inflammatory processes. In cattle, TNF-α is primarily produced by activated macrophages, monocytes, dendritic cells, and T lymphocytes in response to infection, endotoxin exposure, or tissue injury. TNF-α exerts its biological effects by binding to TNF receptors TNFR1 (p55) and TNFR2 (p75), activating intracellular signaling pathways including NF-κB, MAPK, and caspase-mediated pathways, which regulate cytokine production, immune cell activation, apoptosis, and inflammatory responses. In bovine health, TNF-α plays an important role in immune responses to bacterial infections such as mastitis caused by Escherichia coli or Staphylococcus aureus, as well as mycobacterial infections including bovine tuberculosis (Mycobacterium bovis), where it contributes to macrophage activation and granuloma formation. TNF-α is also involved in systemic inflammatory responses and metabolic changes during infection or stress. Because TNF-α is a key regulator of inflammation and host defense, bovine TNF-α is widely studied in veterinary immunology, infectious disease research, and mastitis biology, contributing to improved understanding of immune responses and inflammatory regulation in cattle.

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