Equine CCL3 (MIP-1 alpha) Recombinant Protein
- Catalog Number:
- RP0065E
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Equus caballus (horse), Equus przewalskii (Przewalski's horse)
- Equine CCL3 (alias MIP-1α, macrophage inflammatory protein-1 alpha) (catalog RP0065E) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~7.9 kDa, 70 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across horse and Przewalski's horse. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used to study chemokine signaling and leukocyte chemoattraction/migration; typical experimental uses include chemotaxis and cell-migration assays, cell-culture stimulation and dosing studies, ELISA and neutralization assays, receptor-binding studies, flow-cytometry and Western blot controls, and antibody generation/validation.
| Amino Acid Sequence | VPFGADTPTA CCFSYVSRQI PRKFINDYYE TSSQCSKPAI IFQTKRSRQV CADPSEAWVQ EYVTDLELSA (70) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 7.8 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Equine CCL3 (C-C motif chemokine ligand 3, also known as MIP-1α, macrophage inflammatory protein-1 alpha) is a pro-inflammatory chemokine that regulates recruitment and activation of immune cells during inflammatory and infectious responses in horses (Equus caballus). CCL3 primarily signals through the CCR1 and CCR5 receptors, promoting chemotaxis of monocytes, macrophages, dendritic cells, natural killer (NK) cells, and activated T lymphocytes to sites of infection or tissue injury. In horses, CCL3 is produced by activated macrophages, epithelial cells, endothelial cells, and lymphocytes in response to inflammatory stimuli such as microbial components and cytokines including TNF-α and IL-1β. CCL3-mediated immune cell recruitment contributes to host defense in conditions such as equine respiratory infections (including equine influenza and bacterial pneumonia), inflammatory airway disease, and musculoskeletal inflammation, where infiltration of monocytes and T cells supports immune activation and tissue repair. However, excessive or prolonged CCL3 expression may contribute to persistent inflammation and tissue damage in chronic inflammatory conditions such as equine asthma or joint inflammation. As a regulator of leukocyte trafficking and inflammatory signaling, equine CCL3 is important in studies of host-pathogen interactions, respiratory disease, and inflammatory mechanisms affecting equine health and performance.
