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Equine CCL11 (Eotaxin-1) Recombinant Protein
- Catalog Number:
- RP0066E
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Equus asinus (ass), Equus caballus (horse), Equus przewalskii (Przewalski's horse), Equus quagga (plains zebra)
- Equine CCL11 (alias Eotaxin, also known as Eotaxin-1) (catalog RP0066E) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~9.0 kDa, 78 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across several equid species: horse, donkey, Przewalski's horse, and plains zebra. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used to study chemokine signaling and leukocyte chemoattraction/migration; typical experimental uses include chemotaxis and cell-migration assays, cell-culture stimulation and dosing studies, ELISA and neutralization assays, receptor-binding studies, flow-cytometry and Western blot controls, and antibody generation/validation.
| Amino Acid Sequence | AQPVSISTVC CFNVASRKIS FQRLQSYRKI TSSKCPQKAV IFKTKQAKKI CADPKQKWVQ DAMKYLDENS RTTKYSSF (78) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 9.0 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Equine CCL11 (C-C motif chemokine ligand 11), also known as eotaxin-1, is a member of the CC chemokine family that regulates eosinophil recruitment and type 2-associated immune responses in horses (Equus caballus). CCL11 is produced primarily by epithelial cells, fibroblasts, endothelial cells, and activated immune cells in response to Th2-associated cytokines such as IL-4 and IL-13, and it functions predominantly through the CCR3 receptor to promote chemotaxis and activation of eosinophils, as well as certain Th2 lymphocytes and basophils. In healthy horses, basal CCL11 expression is generally low, but levels may increase during allergic airway disease (equine asthma), parasitic infections, hypersensitivity reactions, and inflammatory conditions affecting the respiratory or gastrointestinal tract. In veterinary and comparative medicine research, equine CCL11 is particularly relevant in studies of eosinophilic inflammation, airway hyperresponsiveness, parasite immunity, and Th2-skewed immune responses. Characterization of CCL11 in horses provides valuable insight into chemokine-mediated leukocyte trafficking, allergic and parasitic disease mechanisms, and immune regulation in equine health and translational respiratory research.
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