Slide 1 of 1 
Swine CCL4 (MIP-1 beta) Recombinant Protein
- Catalog Number:
- RP0069S
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Sus scrofa (pig)
- Swine CCL4 (alias MIP-1β, macrophage inflammatory protein-1 beta) (catalog RP0069S) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~7.8 kDa, 69 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to pig. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used to study chemokine signaling and leukocyte chemoattraction/migration; typical experimental uses include chemotaxis and cell-migration assays, cell-culture stimulation and dosing studies, ELISA and neutralization assays, receptor-binding and signaling studies, flow-cytometry and Western blot controls, and antibody generation/validation.
| Amino Acid Sequence | APMGSDPPTS CCFTYTVRKL PRNFVTDYYE TSSLCSQPAV VFQTKKGRQV CANPSDDWVQ EYMDDLELN (69) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 7.8 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Swine CCL4 (C-C motif chemokine ligand 4, also known as MIP-1β, macrophage inflammatory protein-1 beta) is a pro-inflammatory chemokine that regulates recruitment and activation of immune cells during inflammatory and infectious responses in pigs (Sus scrofa domesticus). CCL4 primarily signals through the CCR5 receptor, promoting chemotaxis of monocytes, macrophages, dendritic cells, natural killer (NK) cells, and activated T lymphocytes to sites of infection, inflammation, or tissue injury. In pigs, CCL4 is produced by activated macrophages, dendritic cells, epithelial cells, and lymphocytes in response to inflammatory stimuli such as pathogen-associated molecules and cytokines including TNF-α and IL-1β. CCL4-mediated immune cell recruitment is particularly relevant in respiratory and systemic infectious diseases, including porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza virus, and bacterial pathogens involved in porcine respiratory disease complex, where immune cell trafficking to infected tissues contributes to antiviral and antibacterial defense. While CCL4-driven leukocyte migration supports pathogen clearance and immune activation, excessive or prolonged expression may contribute to inflammatory tissue damage, particularly in lung tissue during severe respiratory infections. Because pigs are widely used as large-animal models for human infectious and inflammatory diseases, characterization of swine CCL4 supports studies of chemokine-mediated immune cell migration, host-pathogen interactions, and development of vaccines and immunomodulatory therapies relevant to both veterinary medicine and translational biomedical research.
Please choose a size to see price
