Bovine CCL11 (Eotaxin-1) Recombinant Protein

Catalog Number:: RP0071B
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Bison bison bison (American buffalo), Bos indicus (zebu), Bos javanicus (banteng), Bos mutus (wild yak), Bos taurus (cattle), Bubalus bubalis (water buffalo), Bubalus kerabau (carabao)

Bovine CCL11 (alias Eotaxin, also known as Eotaxin-1) (catalog RP0071B) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~8.6 kDa, 74 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across several bovine species (cattle, American buffalo, zebu, banteng, wild yak, water buffalo, and carabao). Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used to study chemokine signaling and leukocyte chemoattraction/migration (particularly eosinophil recruitment); typical experimental uses include chemotaxis and cell-migration assays, cell-culture stimulation and dosing studies, ELISA and neutralization assays, receptor-binding studies, flow-cytometry and Western blot controls, and antibody generation/validation.

Amino Acid Sequence	QPASIPTICC FNMSKKKISI QRLQSYRKIT SSKCPQKAVI FNTKQNKKIC VDPQEKWVQN AMEYLNQKSQ TLKS (74)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	8.6 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Bovine CCL11 (C-C motif chemokine ligand 11), also known as eotaxin-1, is a member of the CC chemokine family that regulates eosinophil recruitment and type 2-associated immune responses in cattle (Bos taurus). CCL11 is produced primarily by epithelial cells, fibroblasts, endothelial cells, and activated immune cells in response to Th2-associated cytokines such as IL-4 and IL-13, and it functions mainly through the CCR3 receptor to promote chemotaxis and activation of eosinophils, as well as certain Th2 lymphocytes and basophils. In healthy cattle, basal CCL11 expression is typically low, but levels may increase during parasitic infections-particularly gastrointestinal nematode and lungworm infections-as well as allergic-type inflammation, mastitis-associated immune responses, and respiratory disease. In veterinary and agricultural research, bovine CCL11 serves as a biomarker of Th2-skewed immunity and eosinophilic inflammation, providing insight into host defense against helminths, vaccine responses, and immune regulation in cattle.

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