Bovine IL-21 Recombinant Protein

Catalog Number:: RP0074B
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Bos taurus (cattle)

Bovine IL-21 (catalog RP0074B) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~15.1 kDa, 129 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to cattle. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used in immunology and cell-biology research to study IL-21 signaling and its effects on B-cell differentiation, T-cell and NK-cell function, and antibody responses; typical experimental uses include cell-culture stimulation and dosing studies, proliferation/differentiation assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation.

Amino Acid Sequence	HKSSSQGQDR LFIRLRQLID IVDQLKNYVN DLDPEFLPAP EDVKRHCERS AFSCFQKVQL KSANNGDNEK IINILTKQLK RKLPATNTGR RQKHEVTCPS CDSYEKKPPK EYLERLKSLI QKMIHQHLS (129)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	15.1 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Bovine IL-21 (Interleukin-21) is a pleiotropic cytokine belonging to the common γ chain (γc) family that plays a central role in regulation of adaptive immunity in cattle (Bos taurus). IL-21 is produced primarily by activated CD4⁺ T cells, particularly T follicular helper (Tfh) cells and Th17 cells, following antigen stimulation. It signals through the IL-21 receptor (IL-21R) in association with the common γ chain, activating downstream pathways such as JAK/STAT (predominantly STAT3) to modulate gene expression in target cells. IL-21 promotes B cell proliferation, differentiation, class-switch recombination, and antibody production, supports CD8⁺ T cell cytotoxic function, and influences NK cell activity, while also contributing to regulation of Th17 responses. In healthy cattle, basal IL-21 expression is low but increases during infection, vaccination, and immune activation. Bovine IL-21 has been implicated in immune responses to pathogens such as bovine viral diarrhea virus (BVDV), bovine leukemia virus (BLV), Mycobacterium bovis, and agents of bovine respiratory disease complex. In veterinary and agricultural research, IL-21 serves as a key biomarker of T cell-dependent B cell help and humoral immunity and is studied to evaluate vaccine efficacy, host-pathogen interactions, and adaptive immune regulation in cattle.

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