Swine CCL3L1 Recombinant Protein

Catalog Number:: RP0075S
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Sus scrofa (pig)

Product Description

Swine CCL3L1 (alias MIP-1α-like, macrophage inflammatory protein-1 alpha-like) (catalog RP0075S) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~7.8 kDa, 70 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to pig. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used to study chemokine signaling and leukocyte chemoattraction/migration; typical experimental uses include chemotaxis and cell-migration assays, receptor-binding and signaling studies, cell-culture stimulation and dosing studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation.

Specifications

Amino Acid Sequence	APLGADTPTA CCFSYTSRQL PRKFVADYFE TSSQCSKPGV IFQTKKGREV CANPEDAWVQ EYISDLELNA (70)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	7.8 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Swine CCL3L1 (C-C motif chemokine ligand 3-like 1, also known as LD78β or MIP-1αP) is a chemokine closely related to CCL3 (MIP-1α) that plays a role in leukocyte recruitment and regulation of inflammatory immune responses in pigs (Sus scrofa domesticus). CCL3L1 primarily signals through the CCR5 receptor, and to a lesser extent CCR1, promoting chemotaxis of monocytes, macrophages, dendritic cells, natural killer (NK) cells, and activated T lymphocytes to sites of infection or tissue injury. In swine, CCL3L1 is produced by activated macrophages, dendritic cells, and lymphocytes in response to inflammatory stimuli such as pathogen-associated molecular patterns and cytokines including TNF-α and IL-1β. CCL3L1-mediated signaling contributes to host defense by coordinating immune cell trafficking and amplifying inflammatory responses during infections, including porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza virus, and bacterial respiratory pathogens involved in porcine respiratory disease complex. Because CCR5-mediated chemokine signaling influences antiviral immunity and immune cell activation, CCL3L1 may also participate in regulation of inflammatory lung responses during respiratory infections. As a regulator of leukocyte migration and inflammatory signaling, swine CCL3L1 is relevant in studies of host-pathogen interactions, immune regulation, and vaccine responses, and contributes to understanding chemokine networks that influence disease resistance and immune function in pigs.

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