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Bovine IL-36 Receptor Antagonist (IL-36RA) Recombinant Protein
- Catalog Number:
- RP0076B
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Bos indicus (zebu), Bos taurus (cattle)
- Bovine IL-36 receptor antagonist (aliases IL-36RA, IL-1F5) (catalog RP0076B) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~17.1 kDa (calculated), 155 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across cattle and zebu. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used to study IL-36/IL-1 family signaling, IL-36 receptor antagonism and regulation of inflammatory responses; typical experimental uses include cell-culture stimulation and dosing studies, cytokine signaling and inhibition assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation.
| Amino Acid Sequence | MVLSGALCFR MKDAALKVLY LHDNQLLAGG LQAGKVIKGE EISVVPNRSL DAKLSPVILG VHGGSQCLSC GTGQEPTLKL EPVNIMELYH SAEKSKKFTF YRRDTGLTSS FESAAYPGWF LCTVPEADQP LQITQLPKDT SWDNPIIDFY FQQCD (155) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 17.1kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Bovine Interleukin-36 Receptor Antagonist (IL-36Ra) is an anti-inflammatory cytokine that regulates IL-36-mediated immune responses in cattle (Bos taurus). IL-36Ra is a member of the IL-1 cytokine family and functions as a natural inhibitor of the IL-36 signaling pathway by binding to the IL-36 receptor (IL-36R, also known as IL1RL2) without activating it, thereby preventing proinflammatory cytokines such as IL-36α, IL-36β, and IL-36γ from initiating downstream signaling. Through this mechanism, bovine IL-36Ra suppresses activation of intracellular pathways including NF-κB and MAPK, helping to limit production of inflammatory mediators and regulate immune responses at epithelial barrier tissues such as the skin, respiratory tract, and gastrointestinal mucosa. In cattle, IL-36Ra contributes to maintaining immune homeostasis and controlling excessive inflammatory responses during infection or tissue injury. In cell culture, recombinant bovine IL-36Ra is commonly used to inhibit IL-36-induced inflammatory signaling in epithelial and immune cells, allowing researchers to study cytokine regulation, epithelial-immune interactions, and modulation of inflammatory pathways in vitro. Bovine IL-36Ra is also studied in veterinary immunology and translational research to better understand mechanisms of epithelial inflammation, host-pathogen interactions, and immune regulation relevant to livestock health and comparative inflammatory disease biology.
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