Bovine IL-12/IL-23 p40 Recombinant Protein

Catalog Number:: RP0077B
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Bos taurus (cattle)

Product Description

Bovine IL-12 p40 (alias IL-12/IL-23 p40) (catalog RP0077B) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~34.6 kDa (calculated), 305 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to cattle. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used to study IL-12/IL-23 biology and signaling, dendritic-cell and T-cell polarization (Th1/Th17 responses), cytokine biology and receptor interactions; typical experimental uses include cell-culture stimulation and dosing studies, polarization and functional assays, ELISA and neutralization assays, receptor-binding and signaling studies, flow-cytometry and Western blot controls, and antibody generation/validation.

Specifications

Amino Acid Sequence	MWELEKNVYV VELDWYPDAP GETVVLTCDT PEEDGITWTS DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEALSRSLL LLHKKEDGIW STDILKDQKE PKAKSFLKCE AKDYSGHFTC WWLTAISTDL KFSVKSSRGS SDPRGVTCGA ALLSAEKVSL EHREYNKYTV ECQEGSACPA AEESLLIEVV VEAVHKLKYE NYTSSFFIRD IIKPDPPKNL QLRPLKNSRQ VEVSWEYPDT WSTPHSYFSL TFCVQVQGKN KREKKLFMDQ TSAKVTCHKD ANVRVQARDR YYSSFWSEWA SVSCS (305)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	34.6kda
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Bovine Interleukin-12/Interleukin-23 p40 (IL-12/IL-23 p40) is a cytokine subunit that plays an important role in regulating immune responses in cattle (Bos taurus). The p40 subunit is shared by two heterodimeric cytokines, IL-12 and IL-23, where it pairs with the p35 subunit to form IL-12 and with the p19 subunit to form IL-23. These cytokines are produced primarily by activated antigen-presenting cells, including macrophages and dendritic cells, in response to microbial infection or inflammatory stimuli. Bovine IL-12/IL-23 p40 contributes to activation and differentiation of T helper cells and natural killer (NK) cells, promoting Th1-type immune responses and production of interferon-γ (IFN-γ), which are critical for defense against intracellular pathogens. Through IL-23 signaling, the p40 subunit also supports the maintenance and expansion of Th17 cells involved in inflammatory responses and host defense. In cattle, IL-12/IL-23 p40 is involved in immune responses against bacterial, viral, and parasitic infections, including important livestock pathogens such as Mycobacterium bovis, Salmonella spp., and Mycobacterium avium subsp. paratuberculosis. Due to these functions, bovine IL-12/IL-23 p40 is widely studied in veterinary immunology and translational research, particularly in models of bovine tuberculosis and Johne's disease, where cytokine responses are used to investigate host immunity, evaluate vaccine candidates, and develop diagnostic and immunomodulatory strategies relevant to both animal health and comparative biomedical research.

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