Bovine CXCL10 (IP-10) Recombinant Protein

Catalog Number:: RP0079B
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Bison bison bison (American buffalo), Bos grunniens (domestic yak), Bos indicus (zebu), Bos javanicus (banteng), Bos mutus (wild yak), Bos taurus (cattle)

Bovine CXCL10 (alias IP-10, interferon-gamma-inducible protein 10) (catalog RP0079B) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~9.3 kDa, 83 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across several bovine species (cattle, American buffalo, domestic yak, zebu, banteng, and wild yak). Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used to study chemokine signaling and leukocyte chemoattraction/migration (particularly CXCR3⁺ T cells and NK cells), inflammation and immune-cell trafficking; typical experimental uses include chemotaxis and cell-migration assays, receptor-binding and signaling studies, cell-culture stimulation and dosing studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation.

Amino Acid Sequence	VPLSRNTRCS CIEISNGSVN PRSLEKLEVI PASQSCPRVE IIATMKKNGE KRCLNPESKT IKNLLKAINK QRTKRSPRTR KEA (83)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	14.9 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Bovine CXCL10 (C-X-C motif chemokine ligand 10), also known as IP-10 (Interferon gamma-induced protein 10), is a proinflammatory chemokine belonging to the CXC chemokine family, which also includes CXCL9 (MIG) and CXCL11 (I-TAC) that regulate T-cell recruitment during antiviral and inflammatory immune responses. In cattle (Bos taurus), CXCL10 is produced by macrophages, dendritic cells, endothelial cells, epithelial cells, and fibroblasts in response to interferon-γ (IFN-γ), viral infection, or inflammatory cytokines. CXCL10 signals primarily through the chemokine receptor CXCR3, which is expressed on activated T lymphocytes, natural killer (NK) cells, and other immune cells, promoting chemotaxis and activation of these cells at sites of infection or inflammation. In bovine health, CXCL10 is involved in immune responses to viral and bacterial pathogens, including bovine viral diarrhea virus (BVDV) and Mycobacterium bovis, the causative agent of bovine tuberculosis, where CXCL10-mediated recruitment of Th1-type T cells contributes to granuloma formation and cellular immune responses. Because bovine tuberculosis closely resembles human tuberculosis in terms of immune pathology and granulomatous inflammation, cattle are used as comparative animal models for tuberculosis research, and CXCL10 serves as an important marker of IFN-γ-driven immune responses and T-cell trafficking in studies of host-pathogen interactions, diagnostics, and vaccine development.

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