Swine TNF alpha Recombinant Protein

Catalog Number:: RP0080S
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Sus scrofa (pig)

Product Description

Swine TNF-α (catalog RP0080S) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~16.9 kDa, 154 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to pig. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used in immunology and cell-biology research to study TNF-α signaling, inflammation, apoptosis and cytokine biology; typical experimental uses include cell-culture stimulation and dosing studies, cytotoxicity and apoptosis assays, ELISA and neutralization assays, receptor-binding studies, flow-cytometry and Western blot controls, and antibody generation/validation.

Specifications

Amino Acid Sequence	SSSQTSDKPV AHVVANVKAE GQLQWQSGYA NALLANGVKL KDNQLVVPTD GLYLIYSQVL FRGQGCPSTN VFLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKDDRLS AEINLPDYLD FAESGQVYFG IIAL (154)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	34.6 kda
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Swine Tumor Necrosis Factor Alpha (TNF-α) (also known as TNF or cachectin) is a proinflammatory cytokine that plays a central role in innate immune responses, inflammation, and host defense against pathogens in pigs (Sus scrofa). TNF-α is a member of the tumor necrosis factor (TNF) superfamily, which includes related cytokines such as TNF-β (lymphotoxin-α), Fas ligand (FasL), CD40 ligand (CD40L), and TRAIL, molecules that regulate immune signaling, apoptosis, and inflammatory pathways. In pigs, TNF-α is primarily produced by activated macrophages, monocytes, dendritic cells, and T lymphocytes in response to infection, endotoxin exposure, or tissue injury. TNF-α exerts its biological effects by binding to TNF receptors TNFR1 (p55) and TNFR2 (p75), activating intracellular signaling pathways including NF-κB, MAPK, and caspase-mediated pathways, which regulate cytokine production, immune cell activation, apoptosis, and inflammatory responses. In swine health, TNF-α is involved in immune responses to bacterial, viral, and parasitic infections, including porcine reproductive and respiratory syndrome virus (PRRSV), African swine fever virus (ASFV), and bacterial respiratory pathogens, where it contributes to inflammatory defense and activation of innate immunity. However, excessive TNF-α production can contribute to systemic inflammation, fever, and tissue damage during severe infections. Because pigs are widely used as large-animal models for human immunology and infectious disease, swine TNF-α is extensively studied in veterinary immunology, respiratory disease research, and translational biomedical models, providing insights into cytokine regulation and inflammatory responses relevant to both animal health and human disease.

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