Canine IL-1 beta Recombinant Protein

Catalog Number:: RP0085D
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Canis lupus dingo (dingo), Canis lupus familiaris (dog)

Product Description

Canine IL-1β (catalog RP0085D) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~17.5 kDa, 152 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to dog and dingo. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used to study IL-1β signaling, inflammation, inflammasome/pyroptosis and innate immune responses; typical experimental uses include cell-culture stimulation and dosing studies, cytokine and inflammasome assays, ELISA and neutralization assays, receptor-binding and signaling studies, flow-cytometry and Western blot controls, and antibody generation/validation.

Specifications

Amino Acid Sequence	AAMQSVDCKL QDISHKYLVL SNSYELRALH LNGENVNKQV VFHMSFVHGD ESNNKIPVVL GIKQKNLYLS CVMKDGKPTL QLEKVDPKVY PKRKMEKRFV FNKIEIKNTV EFESSQYPNW YISTSQVEGM PVFLGNTRGG QDITDFTMEF SS (152)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	14.9 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Canine Interleukin-1 Beta (IL-1β) is a potent pro-inflammatory cytokine produced primarily by activated macrophages, monocytes, and dendritic cells in dogs (Canis lupus familiaris) following recognition of pathogens or tissue damage. Synthesized as an inactive precursor (pro-IL-1β), it is cleaved into its active form by caspase-1 within the inflammasome complex and subsequently binds to the IL-1 receptor (IL-1R1), activating NF-κB and MAPK signaling pathways that drive fever, leukocyte recruitment, endothelial activation, and acute-phase protein production. In canine health, IL-1β plays a central role in inflammatory and infectious conditions including bacterial pneumonia, pyometra, parvoviral enteritis, osteoarthritis, immune-mediated diseases, and sepsis, where excessive or sustained IL-1β production can contribute to tissue damage and systemic inflammation. Elevated IL-1β levels are also associated with chronic inflammatory disorders and certain cancers, reflecting its role in shaping the tumor microenvironment. As both a biomarker and therapeutic target, canine IL-1β is important in veterinary medicine for monitoring inflammatory status and evaluating anti-inflammatory interventions. In translational research, dogs serve as valuable spontaneous models for human osteoarthritis, inflammatory bowel disease, sepsis, and cancer, and characterization of canine IL-1β supports studies of inflammasome activation, cytokine-driven pathology, and development of targeted immunomodulatory therapies relevant to both canine and human health.

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