Canine IL-8 Recombinant Protein

Catalog Number:: RP0101D
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Canis lupus dingo (dingo), Canis lupus familiaris (dog), Nyctereutes procyonoides (raccoon dog), Vulpes lagopus (Arctic fox), Vulpes vulpes (red fox)

Product Description

Canine IL-8 (alias CXCL8, interleukin-8) (catalog RP0101D) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~8.6 kDa, 74 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across several canids: dog, dingo, raccoon dog, Arctic fox, and red fox. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used to study chemokine signaling, neutrophil and leukocyte chemoattraction/activation, and inflammation; typical experimental uses include chemotaxis and cell-migration assays, cell-culture stimulation and dosing studies, ELISA and neutralization assays, receptor-binding and signaling studies, flow-cytometry and Western blot controls, and antibody generation/validation.

Specifications

Amino Acid Sequence	VSSELRCQCI KTHSTPFHPK YIKELRVIDS GPHCENSEII VKLFNGNEVC LDPKEKWVQK VVQIFLKKAE KQDP (74)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	8.6kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Canine Interleukin-8 (IL-8), also known as CXCL8, is a potent pro-inflammatory chemokine that plays a central role in innate immune responses in dogs (Canis lupus familiaris) by mediating neutrophil chemotaxis, activation, and migration to sites of infection or tissue injury. Produced by macrophages, epithelial cells, endothelial cells, and fibroblasts in response to microbial stimuli and inflammatory cytokines, canine IL-8 signals primarily through CXCR1 and CXCR2 receptors to activate downstream pathways that promote leukocyte recruitment and amplification of acute inflammation. Elevated IL-8 expression is associated with a variety of canine diseases, including bacterial pneumonia, canine influenza, parvoviral enteritis, pyometra, atopic dermatitis, osteoarthritis, and inflammatory bowel disease, where excessive neutrophilic infiltration can contribute to tissue damage and chronic inflammation. As a biomarker, IL-8 is useful for assessing inflammatory status and monitoring therapeutic response in clinical veterinary medicine. Importantly, dogs serve as valuable spontaneous animal models for human diseases characterized by neutrophil-driven pathology, including asthma (canine chronic bronchitis), inflammatory bowel disease, atopic dermatitis, osteoarthritis, sepsis, and certain cancers such as osteosarcoma and lymphoma. Characterization of canine IL-8 in these naturally occurring models enhances translational research into chemokine-mediated immune cell trafficking, chronic inflammation, tumor microenvironment biology, and the development of targeted anti-inflammatory and immunomodulatory therapies relevant to both canine and human health.

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