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Canine IL-6 Recombinant Protein
- Catalog Number:
- RP0103D
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Canis lupus dingo (dingo), Canis lupus familiaris (dog)
- Canine IL-6 (catalog RP0103D) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~21.0 kDa, 187 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to dog and dingo. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used to study IL-6 signaling, acute-phase and inflammatory responses, and B-cell differentiation; typical experimental uses include cell-culture stimulation and dosing studies, proliferation/differentiation assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation.
| Amino Acid Sequence | FPTPGPLAGD SKDDATSNSL PLTSANKVEE LIKYILGKIS ALRKEMCDKF NKCEDSKEAL AENNLHLPKL EGKDGCFQSG FNQETCLTRI TTGLVEFQLH LNILQNNYEG DKENVKSVHM STKILVQMLK SKVKNQDEVT TPDPTTDASL QAILQSQDEW LKHTTIHLIL RSLEDFLQFS LRAVRIM (187) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 21.0kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Canine Interleukin-6 (IL-6) is a multifunctional pro-inflammatory cytokine and a central member of the IL-6 cytokine family-including IL-6, IL-11, IL-27, IL-31, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1), and cardiotrophin-like cytokine factor 1 (CLCF1)-all of which signal through receptor complexes utilizing the shared gp130 subunit to activate JAK/STAT and MAPK pathways. In dogs (Canis lupus familiaris), IL-6 regulates acute-phase protein production (such as C-reactive protein and serum amyloid A), B- and T-cell differentiation, hematopoiesis, and systemic inflammatory responses. Elevated IL-6 levels are associated with infectious diseases including canine parvovirus, distemper, and sepsis, as well as chronic inflammatory and immune-mediated disorders, osteoarthritis, and neoplasia. Importantly, several naturally occurring canine diseases serve as translational models for human conditions in which IL-6 plays a pathogenic role, including canine osteosarcoma (model for pediatric osteosarcoma), lymphoma (model for non-Hodgkin lymphoma), melanoma, atopic dermatitis (model for allergic skin disease), inflammatory bowel disease (model for human IBD), rheumatoid-like immune-mediated polyarthritis, and dilated cardiomyopathy. Dysregulated IL-6 signaling in these conditions contributes to inflammation, tumor progression, and systemic pathology, making canine IL-6 a critical biomarker and therapeutic target in veterinary medicine and a valuable component of comparative and translational research.
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