Slide 1 of 1 
Equine IL-5 Recombinant Protein
- Catalog Number:
- RP0104E
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Equus caballus (horse)
- Equine IL-5 (catalog RP0104E) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~12.9 kDa, 114 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to horse. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used to study IL-5 signaling, eosinophil proliferation, activation and survival, and Th2/eosinophilic responses; typical experimental uses include cell-culture stimulation and dosing studies, proliferation/activation assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation.
| Amino Acid Sequence | AVESPMNRLV AETLTLLSTH RTLLIGDGNL MIPTPEHKNH QLCIEEVFQG IDTLKNQTVQ GDAVAKLFQN LSLIKGYIDL QKKKCGGERW RVKQFLDYLQ EFLGVINTEW TIEG (114) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 12.9kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Equine Interleukin-5 (IL-5) is a Th2-associated cytokine produced primarily by activated CD4⁺ T helper 2 (Th2) cells, mast cells, and type 2 innate lymphoid cells in horses (Equus caballus), where it plays a central role in eosinophil differentiation, activation, recruitment, and survival. IL-5 is a member of the common beta chain (βc) cytokine family, which also includes IL-3 and granulocyte-macrophage colony-stimulating factor (GM-CSF); these cytokines share the βc receptor subunit and regulate hematopoiesis and myeloid cell function. IL-5 signals through a receptor complex composed of IL-5Rα and the shared βc chain, activating JAK/STAT and MAPK pathways that promote eosinophil maturation in the bone marrow and enhance their effector activity in peripheral tissues. In equine health, IL-5 is particularly relevant in eosinophil-associated conditions such as equine asthma (recurrent airway obstruction), insect bite hypersensitivity ("sweet itch"), parasitic infections, and certain gastrointestinal inflammatory disorders, where elevated IL-5 contributes to eosinophilic infiltration, airway inflammation, pruritic dermatitis, and tissue remodeling. While IL-5-mediated responses support protective immunity against helminths, excessive or chronic IL-5 signaling can drive persistent allergic inflammation and fibrosis. As a biomarker of type 2 immune activation and eosinophilic disease, equine IL-5 is important in allergy research, vaccine response studies, and immune modulation strategies. In translational research, horses serve as valuable large-animal models for human asthma and allergic airway disease, and characterization of equine IL-5 supports comparative investigations into βc cytokine signaling and development of targeted anti-IL-5 therapies relevant to both veterinary and human medicine.
Please choose a size to see price
RELATED MEDIA
