Bovine IL-1 beta Recombinant Protein

Catalog Number:: RP0106B
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Bos indicus (zebu), Bos javanicus (banteng), Bos taurus (cattle)

Bovine IL-1β (catalog RP0106B) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~17.7 kDa, 153 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across several bovine species (cattle, zebu, and banteng). Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used to study IL-1β signaling, inflammation, inflammasome/pyroptosis and innate immune responses; typical experimental uses include cell-culture stimulation and dosing studies, cytokine and inflammasome assays, ELISA and neutralization assays, receptor-binding and signaling studies, flow-cytometry and Western blot controls, and antibody generation/validation.

Amino Acid Sequence	APVQSIKCKL QDREQKSLVL ASPCVLKALH LLSQEMNREV VFCMSFVQGE ERDNKIPVAL GIKDKNLYLS CVKKGDTPTL QLEEVDPKVY PKRNMEKRFV FYKTEIKNTV EFESVLYPNW YISTSQIEER PVFLGHFRGG QDITDFRMET LSP (153)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	17.7 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Bovine Interleukin-1 Beta (IL-1β) is a potent pro-inflammatory cytokine produced primarily by activated macrophages, monocytes, and dendritic cells in cattle (Bos taurus) in response to microbial components or tissue injury. Synthesized as an inactive precursor (pro-IL-1β), it requires cleavage by caspase-1 within the inflammasome complex to become biologically active, after which it binds to the IL-1 receptor (IL-1R1) and activates NF-κB and MAPK signaling pathways to promote fever, leukocyte recruitment, endothelial activation, and acute-phase protein production. In bovine health, IL-1β plays a central role in inflammatory responses associated with economically significant diseases such as mastitis, bovine respiratory disease (BRD), metritis, lameness, and enteric infections, where it contributes to both pathogen clearance and inflammatory tissue damage. Elevated IL-1β expression in the mammary gland is closely linked to neutrophil recruitment and severity of intramammary infection. As a biomarker of innate immune activation, bovine IL-1β is widely used in studies of vaccine responses, immune modulation, and disease pathogenesis. In veterinary and translational research, characterization of bovine IL-1β supports investigations into inflammasome biology, host-pathogen interactions, inflammatory regulation in large-animal systems, and development of strategies to mitigate excessive inflammation while preserving protective immunity.

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