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Canine IL-5 Recombinant Protein
- Catalog Number:
- RP0117D
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Canis lupus dingo (dingo), Canis lupus familiaris (dog)
- Canine IL-5 (catalog RP0117D) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~13.1 kDa, 113 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to dog and dingo. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used to study IL-5 signaling, eosinophil proliferation, activation and survival, and Th2/eosinophilic responses; typical experimental uses include cell-culture stimulation and dosing studies, proliferation/activation assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation.
| Amino Acid Sequence | VENPMNRLVA ETLTLLSTHR TWLIGDGNLM IPTPENKNHQ LCIKEVFQGI DTLKNQTAHG EAVDKLFQNL SLIKEHIERQ KKRCAGERWR VTKFLDYLQV FLGVINTEWT PES (113) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 13.1kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Canine Interleukin-5 (IL-5) is a Th2-associated cytokine produced primarily by activated CD4⁺ T helper 2 (Th2) cells, mast cells, and group 2 innate lymphoid cells in dogs (Canis lupus familiaris), where it plays a central role in eosinophil differentiation, activation, recruitment, and survival. IL-5 is a member of the common beta chain (βc) cytokine family, which also includes IL-3 and granulocyte-macrophage colony-stimulating factor (GM-CSF); these cytokines share the βc receptor subunit and regulate hematopoiesis and myeloid cell function. IL-5 signals through a receptor complex composed of IL-5Rα and the shared βc chain, activating JAK/STAT and MAPK pathways that promote eosinophil maturation in the bone marrow and enhance their effector activity in peripheral tissues. In canine health, IL-5 is particularly important in eosinophil-associated diseases such as eosinophilic bronchopneumopathy, atopic dermatitis, food hypersensitivity, parasitic infections, and certain gastrointestinal inflammatory conditions, where elevated IL-5 contributes to eosinophilic infiltration, tissue inflammation, and remodeling. While IL-5-mediated responses support protective immunity against helminths and other parasites, excessive or chronic IL-5 expression can drive persistent inflammation and tissue damage. As a biomarker of type 2 immune activation and eosinophilic disease, canine IL-5 is relevant in allergy research, vaccine evaluation, and immune modulation studies. In translational research, dogs serve as valuable spontaneous models for human asthma and eosinophilic disorders, and characterization of canine IL-5 supports comparative investigations into βc cytokine signaling and development of targeted anti-IL-5 therapeutic strategies relevant to both veterinary and human medicine.
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