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Swine CCL20 Recombinant Protein
- Catalog Number:
- RP0118S
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Sus scrofa (pig)
- Swine CCL20 (alias MIP-3α, also known as LARC) (catalog RP0118S) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~8.1 kDa, 70 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to pig. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used to study chemokine signaling and leukocyte chemoattraction/migration; typical experimental uses include chemotaxis and cell-migration assays, cell-culture stimulation and dosing studies, ELISA and neutralization assays, receptor-binding studies, flow-cytometry and Western blot controls, and antibody generation/validation.
| Amino Acid Sequence | ASNFDCCLRY TDHILHPRFI MGFTQQLANE ACDINAIIFY TKKKLAVCAD PQKIWVKQAV NILSQRVKKM (70) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 8.1 kda |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Swine CCL20 (C-C motif chemokine ligand 20, also known as MIP-3α, macrophage inflammatory protein-3 alpha, or LARC - liver and activation-regulated chemokine) is a pro-inflammatory chemokine involved in mucosal immune defense and leukocyte recruitment in pigs (Sus scrofa domesticus). CCL20 signals specifically through the CCR6 receptor, promoting chemotaxis of immune cells including immature dendritic cells, memory T cells, Th17 cells, regulatory T cells, and certain B cell populations to sites of epithelial infection or inflammation. In swine, CCL20 is produced by epithelial cells, macrophages, fibroblasts, and other immune cells in response to inflammatory stimuli such as microbial components, cytokines (e.g., IL-1β, TNF-α, and IL-17), and tissue injury. It is highly expressed at mucosal surfaces, particularly in the intestinal and respiratory epithelium, where it plays an important role in coordinating immune responses against pathogens. In swine health, CCL20 is especially relevant in enteric infections caused by pathogens such as Salmonella spp. and Escherichia coli, as well as in respiratory diseases including porcine reproductive and respiratory syndrome virus (PRRSV) and swine influenza, where epithelial production of CCL20 recruits antigen-presenting cells and lymphocytes to infected tissues. While CCL20-mediated immune cell trafficking supports pathogen clearance and mucosal immunity, excessive or prolonged expression can contribute to inflammatory tissue damage. Because pigs are widely used as large-animal models for human gastrointestinal and respiratory disease, characterization of swine CCL20 supports studies of mucosal immunity, chemokine-mediated immune cell migration, and development of improved vaccines and disease-control strategies in both veterinary and translational biomedical research.
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