Swine IFN gamma Recombinant Protein

Catalog Number:: RP0126S
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Sus scrofa (pig)

Swine IFN-γ (catalog RP0126S) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~16.8 kDa, 143 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to pig. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used in immunology and cell-biology research to study interferon-γ signaling, macrophage activation, Th1 immune responses, antigen presentation and antiviral/immunoregulatory functions; typical experimental uses include cell-culture stimulation and dosing studies, ELISA and neutralization assays, receptor-binding and signaling studies, flow-cytometry and Western blot controls, and antibody generation/validation.

Amino Acid Sequence	QAPFFKEITI LKDYFNASTS DVPNGGPLFL EILKNWKEES DKKIIQSQIV SFYFKFFEIF KDNQAIQRSM DVIKQDMFQR FLNGSSGKLN DFEKLIKIPV DNLQIQRKAI SELIKVMNDL SPRSNLRKRK RSQTMFQGQR ASK (143)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	16.8 kda
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Swine Interferon-γ (IFN-γ) is a type II interferon cytokine that plays a central role in cell-mediated immunity and regulation of antimicrobial and inflammatory responses in pigs (Sus scrofa). IFN-γ is produced primarily by activated T helper 1 (Th1) cells, cytotoxic T lymphocytes, and natural killer (NK) cells following stimulation by intracellular pathogens or immune signaling molecules such as IL-12 and IL-18. Swine IFN-γ binds to the interferon-γ receptor (IFNGR1/IFNGR2) expressed on many immune and non-immune cells, activating intracellular signaling through the JAK-STAT pathway, particularly STAT1, which induces the expression of numerous interferon-stimulated genes involved in antigen presentation, macrophage activation, and antimicrobial defense. In pigs, IFN-γ is critical for activation of macrophages, enhancement of MHC class I and II expression, and control of intracellular pathogens, including viruses, bacteria, and parasites. Swine IFN-γ is widely studied in veterinary immunology and translational research, particularly in porcine models of infectious disease; for example, IFN-γ responses are commonly measured in studies of African swine fever virus (ASFV) infection and vaccine development, where IFN-γ-producing T cells serve as an important indicator of protective cellular immune responses.

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