Canine IL-2 Recombinant Protein

Catalog Number:: RP0129D
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Canis lupus dingo (dingo), Canis lupus familiaris (dog), Nyctereutes procyonoides (raccoon dog), Vulpes lagopus (Arctic fox), Vulpes vulpes (red fox)

Canine IL-2 (catalog RP0129D) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~15.5 kDa, 135 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across several canids: dog, dingo, raccoon dog, Arctic fox, and red fox. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used in immunology and cell-biology research to study T-cell proliferation and activation, NK-cell function and cytokine signaling; typical experimental uses include cell-culture stimulation and dosing studies, proliferation/cytotoxicity assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation.

Amino Acid Sequence	APITSSSTKE TEQQMEQLLL DLQLLLNGVN NYENPQLSRM LTFKFYTPKK ATEFTHLQCL AEELKNLEEV LGLPQSKNVH LTDTKELISN MNVTLLKLKG SETSYNCEYD DETATITEFL NKWITFCQSI FSTLT (135)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	15.5 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Canine IL-2 (Interleukin-2) is a central T cell-derived cytokine that regulates lymphocyte proliferation, differentiation, and immune homeostasis in dogs (Canis lupus familiaris). IL-2 is produced primarily by activated CD4⁺ and CD8⁺ T lymphocytes following antigen recognition and costimulatory signaling, and it functions through the IL-2 receptor complex (IL-2Rα/CD25, IL-2Rβ, and the common γ chain) to promote clonal expansion of antigen-specific T cells, enhance cytotoxic T lymphocyte and natural killer (NK) cell activity, and support development and maintenance of regulatory T cells (Tregs). In healthy dogs, basal IL-2 expression is typically low but increases during infection, vaccination, and immune stimulation. Altered IL-2 responses have been associated with canine distemper virus, leishmaniasis, chronic inflammatory diseases, and immune-mediated disorders. Dogs serve as important translational animal models for human lymphoma and other cancers, atopic dermatitis, autoimmune disease, and certain neuroinflammatory and infectious diseases. In veterinary and comparative medicine research, canine IL-2 is widely used as a biomarker of T cell activation and cell-mediated immunity and has been investigated as an immunotherapeutic agent in oncology and chronic infectious disease settings.

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