Feline IFN gamma Recombinant Protein

Catalog Number:: RP0135F
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Acinonyx jubatus (cheetah), Felis catus (domestic cat), Prionailurus bengalensis (leopard cat), Prionailurus viverrinus (fishing cat), Puma concolor (puma)

Product Description

Feline IFN-γ (catalog RP0135F) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~17.0 kDa, 144 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across several felids: domestic cat, cheetah, puma, leopard cat, and fishing cat. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used in immunology and cell-biology research to study interferon-γ signaling, macrophage activation, Th1 immune responses, antigen presentation and antiviral/immunoregulatory functions; typical experimental uses include cell-culture stimulation and dosing studies, ELISA and neutralization assays, receptor-binding and signaling studies, flow-cytometry and Western blot controls, and antibody generation/validation.

Specifications

Amino Acid Sequence	QAMFFKEIEE LKGYFNASNP DVADGGSLFV DILKNWKEES DKTIIQSQIV SFYLKMFENL KDDDQRIQRS MDTIKEDMLD KLLNTSSSKR DDFLKLIQIP VNDLQVQRKA INELFKVMND LSPRSNLRKR KRSQNLFRGR RASK (144)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	17.0 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Feline Interferon-Gamma (IFN-γ) is a type II interferon produced primarily by activated CD4⁺ and CD8⁺ T lymphocytes as well as natural killer (NK) cells in cats (Felis catus), where it plays a central role in cell-mediated (Th1-type) immunity. IFN-γ signals through the IFN-γ receptor complex (IFNGR1/IFNGR2), activating JAK/STAT1 pathways and inducing genes involved in macrophage activation, enhanced antigen presentation via upregulation of MHC class I and II molecules, and promotion of cytotoxic T-cell responses. In feline health, IFN-γ is critical for host defense against intracellular pathogens and viral infections such as feline leukemia virus (FeLV), feline immunodeficiency virus (FIV), feline infectious peritonitis (FIP; feline coronavirus), Toxoplasma gondii, and mycobacterial infections, where balanced Th1 responses contribute to pathogen control while dysregulation may influence immunopathology or chronic inflammation. Clinically, IFN-γ expression levels serve as indicators of cellular immune competence and disease progression. In animal model research, cats serve as important natural models for human retroviral infection (FIV as a model for HIV), coronavirus-associated immune dysregulation (FIP as a model for severe systemic coronavirus disease), and certain immune-mediated and oncologic conditions. Characterization of feline IFN-γ supports studies of antiviral immunity, T-cell function, vaccine development, and comparative Th1 immune regulation, enhancing translational understanding of cell-mediated immune responses relevant to both veterinary and human medicine.

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