Equine TNF alpha Recombinant Protein

Catalog Number:: RP0137E
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Equus caballus (horse)

Product Description

Equine TNF-α (catalog RP0137E) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~17.1 kDa, 157 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to horse (note: material contains a Phe152 to Leu substitution). Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used to study TNF-α signaling, inflammation, apoptosis and cytokine biology; typical experimental uses include cell-culture stimulation and dosing studies, cytotoxicity and apoptosis assays, ELISA and neutralization assays, receptor-binding studies, flow-cytometry and Western blot controls, and antibody generation/validation.

Specifications

Amino Acid Sequence	LRSSSRTPSD KPVAHVVANP QAEGQLQWLS GRANALLANG VKLTDNQLVV PLDGLYLIYS QVLFKGQGCP STHVLLTHTI SRLAVSYPSK VNLLSAIKSP CHTESPEQAE AKPWYEPIYL GGVFQLEKGD QLSAEINQPN YLDFAESGQV YLGIIAL (157)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	17.1 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Equine Tumor Necrosis Factor Alpha (TNF-α) (also known as TNF or cachectin) is a proinflammatory cytokine that plays a central role in innate immune responses, inflammation, and host defense against pathogens in horses (Equus caballus). TNF-α is a member of the tumor necrosis factor (TNF) superfamily, which includes related cytokines such as TNF-β (lymphotoxin-α), Fas ligand (FasL), CD40 ligand (CD40L), and TRAIL, proteins that regulate immune signaling, apoptosis, and inflammatory pathways. In horses, TNF-α is primarily produced by activated macrophages, monocytes, dendritic cells, and T lymphocytes in response to infection, endotoxin exposure, or tissue injury. TNF-α exerts its biological effects by binding to TNF receptors TNFR1 (p55) and TNFR2 (p75), activating intracellular signaling pathways including NF-κB, MAPK, and caspase-mediated pathways, which regulate cytokine production, immune cell activation, apoptosis, and inflammatory responses. In equine health, TNF-α is an important mediator of inflammatory responses associated with bacterial infections, endotoxemia, and systemic inflammatory conditions, including colic-associated endotoxemia, septicemia, and equine laminitis, where elevated TNF-α levels contribute to vascular inflammation and tissue damage. TNF-α also participates in immune responses to respiratory infections and inflammatory airway diseases. Because TNF-α is a key regulator of inflammation and immune defense, equine TNF-α is widely studied in veterinary immunology, inflammatory disease research, and endotoxemia models, contributing to improved understanding of immune responses and inflammatory regulation in horses.

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