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Canine SCF (Stem Cell Factor) Recombinant Protein
- Catalog Number:
- RP0154D
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Canis lupus dingo (dingo), Canis lupus familiaris (dog)
- Canine SCF (catalog RP0154D) is a yeast-derived growth factor supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~27.7 kDa, 248 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across dog and dingo. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used to study SCF/c-Kit signaling, hematopoietic stem and progenitor cell survival and proliferation, mast-cell growth and differentiation, and hematopoiesis; typical experimental uses include cell-culture stimulation and dosing studies, proliferation and colony-forming assays, mast-cell assays, receptor-binding and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation.
| Amino Acid Sequence | GICGKRVTDD VKDVTKLVAN LPKDYKIALK YVPGMDVLPS HCWISVMVEQ LSVSLTDLLD KFSNISEGLS NYSIIDKLVK IVDDLVECTE GYSFENVKKA PKSPELRLFT PEEFFRIFNR SIDAFKDLET VASKSSECVV SSTLSPDKDS RVSVTKPFML PPVAASSLRN DSSSSNRKAS NSIGDSNLQW AAMALPAFFS LVIGFAFGAL YWKKKQPNLT RTVENIQINE EDNEISMLQE KEREFQEV (248) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 27.7 kda |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Canine Stem Cell Factor (SCF) (also known as KIT ligand, KITLG, mast cell growth factor, or steel factor) is a cytokine and growth factor that plays a critical role in hematopoiesis, stem cell maintenance, and immune cell development in dogs (Canis lupus familiaris). SCF belongs to a group of hematopoietic growth factors that regulate the survival, proliferation, and differentiation of stem and progenitor cells. It exerts its biological effects by binding to the c-Kit receptor (CD117), a receptor tyrosine kinase expressed on hematopoietic stem cells, mast cells, melanocytes, and germ cells, activating intracellular signaling pathways including PI3K/AKT, MAPK, and JAK/STAT that promote cell survival, proliferation, and differentiation. In dogs, SCF is produced by stromal cells, fibroblasts, endothelial cells, and epithelial cells, and exists in both membrane-bound and soluble forms, allowing both localized and systemic regulation of c-Kit-expressing cells. Canine SCF is particularly important for mast cell development and survival, making it highly relevant to canine mast cell tumors, one of the most common skin cancers in dogs, where dysregulated c-Kit signaling contributes to abnormal mast cell proliferation. SCF also plays roles in hematopoietic stem cell maintenance, pigmentation, and reproductive cell development. Because of its central role in c-Kit-mediated signaling and stem cell biology, canine SCF is widely studied in veterinary oncology, hematopoiesis, and regenerative medicine, contributing to understanding of mast cell disease and growth factor-regulated cellular development in dogs.
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