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Canine VEGF-A Recombinant Protein
- Catalog Number:
- RP0252D
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Canis lupus dingo (dingo), Canis lupus familiaris (dog), Nyctereutes procyonoides (raccoon dog), Vulpes lagopus (Arctic fox), Vulpes vulpes (red fox)
- Canine VEGF-A (alias VEGF-A, vascular endothelial growth factor A) (catalog RP0252D) is a yeast-derived growth factor supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~22.0 kDa, 188 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across several canids: dog, dingo, raccoon dog, Arctic fox, and red fox. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used to study angiogenesis, endothelial-cell proliferation, migration, vascular permeability and VEGF-receptor signaling; typical experimental uses include endothelial cell culture stimulation and proliferation/tube-formation assays, receptor-binding and signaling studies, ELISA and neutralization assays, and use as flow-cytometry and Western blot controls or for antibody generation/validation.
| Amino Acid Sequence | APMAGGEHKP HEVVKFMDVY QRSYCRPIET LVDIFQEYPD EIEYIFKPSC VPLMRCGGCC NDEGLECVPT EEFNITMQIM RIKPHQGQHI GEMSFLQHSK CECRPKKDRA RQEKKSIRGK GKGQKRKRKK SRYKPWSVPC GPCSERRKHL FVQDPQTCKC SCKNTDSRCK ARQLELNERT CRCDKPRR (188) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 22.0 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Canine Vascular Endothelial Growth Factor A (VEGF-A) is a member of the vascular endothelial growth factor (VEGF) family, which includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, and placental growth factor (PlGF)-key regulators of angiogenesis, vascular development, and endothelial cell function. In dogs (Canis lupus familiaris), VEGF-A is produced by a variety of cell types including endothelial cells, macrophages, fibroblasts, epithelial cells, and tumor cells, particularly in response to hypoxia, inflammation, or tissue injury. VEGF-A exerts its biological effects primarily through binding to VEGF receptor-1 (VEGFR-1/Flt-1) and VEGF receptor-2 (VEGFR-2/KDR) on endothelial cells, activating intracellular signaling pathways such as MAPK/ERK, PI3K/AKT, and PLCγ, which promote endothelial cell proliferation, migration, vascular permeability, and formation of new blood vessels. In canine physiology, VEGF-A plays important roles in normal vascular development, wound healing, and tissue regeneration. Dysregulated VEGF-A expression is also associated with tumor angiogenesis in canine cancers, including hemangiosarcoma, mammary tumors, mast cell tumors, and osteosarcoma, where increased VEGF signaling supports tumor growth and metastasis by promoting blood vessel formation. Because dogs develop many spontaneous cancers similar to those in humans, canine VEGF-A is widely studied in veterinary oncology, angiogenesis research, and comparative cancer biology, contributing to understanding of vascular growth mechanisms and potential anti-angiogenic therapeutic strategies.
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