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Canine VEGF-A (Yeast-derived Recombinant Protein) - 25 micrograms

RP0252D-025
$300.00
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Vascular endothelial growth factor (VEGF) proteins stimulate vasculogenesis and angiogenesis. They are part of the system that restores the oxygen supply to tissues when blood circulation is inadequate. The normal function of VEGF proteins is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. The VEGF family has six members, including VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and Placental Growth Factor (PGF). Activity of VEGF-A, as its name implies, has been studied mostly on cells of the vascular endothelium, although it does have effects on a number of other cell types (e.g., stimulation monocyte/macrophage migration, neurons, cancer cells, kidney epithelial cells). In vitro, VEGF-A has been shown to stimulate endothelial cell mitogenesis and cell migration. VEGF-A is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor (VPF).

Alternate Names - VEGFA, MVCD1, VEGF, VPF, vascular endothelial growth factor A

VEGF-A Homology Across Species
Canis lupus familiaris (dog) VEGF-A – 100%
Canis lupus dingo (dingo) VEGF-A – 100%
Nyctereutes procyonoides (raccoon dog) VEGF-A – 100%
Vulpes lagopus (Arctic fox) VEGF-A – 100%
Vulpes vulpes (red fox) VEGF-A – 100%
Leptonychotes weddellii (Weddell seal) VEGF-A – 99%
Mustela putorius furo (domestic ferret) VEGF-A – 99%
Odobenus rosmarus divergens (Pacific walrus) VEGF-A – 99%
Acinonyx jubatus (cheetah) VEGF-A – 97%
Camelus bactrianus (Bactrian camel) VEGF-A – 97%
Camelus ferus (Wild Bactrian camel) VEGF-A – 97%
Felis catus (domestic cat) VEGF-A – 97%
Orcinus orca (killer whale) VEGF-A – 97%
Panthera pardus (leopard) VEGF-A – 97%
Physeter catodon (sperm whale) VEGF-A – 97%
More - https://blast.ncbi.nlm.nih.gov/

Canine VEGF-A (Yeast-derived Recombinant Protein) - 25 micrograms
Catalog No.:
RP0252D-025
Quantity:
25 ug
Source:
Canine VEGF-A was produced in yeast and therefore does not have endotoxin, is naturally folded, and post-translationally modified.
MW:
The Canine VEGF-A recombinant protein has a predicted molecular weight of 22.0 kDa.
Protein Sequence:
APMAGGEHKP HEVVKFMDVY QRSYCRPIET LVDIFQEYPD EIEYIFKPSC VPLMRCGGCC NDEGLECVPT EEFNITMQIM RIKPHQGQHI GEMSFLQHSK CECRPKKDRA RQEKKSIRGK GKGQKRKRKK SRYKPWSVPC GPCSERRKHL FVQDPQTCKC SCKNTDSRCK ARQLELNERT CRCDKPRR (188)
Country of Origin:
USA
Applications:
The Canine VEGF-A endotoxin-free recombinant protein can be used in cell culture, as an ELISA Standard, and as a Western Blot Control.

23141169

Interleukin-1β, tumour necrosis factor-α and lipopolysaccharide induce C-type natriuretic peptide from canine aortic endothelial cells.

Osterbur K, Yu DH, Declue AE.

Res Vet Sci. 2012 Nov 8. doi:pii: S0034-5288(12)00305-0. 10.1016/j.rvsc.2012.10.002.

Applications: Stimulation of canine aortic endothelial cells

Abstract
The N-terminal portion of pro C-type natriuretic peptide (NT-pCNP) has shown promise as a biomarker for sepsis in humans and dogs, however the mechanism of NT-pCNP production in dogs is unknown. Canine aortic endothelial cells were stimulated with lipopolysaccharide, lipoteichoic acid, peptidoglycan, TNF-α, IL-1β, IL-6, IL-10, IL-21, CXCL-8, IFN-γ, VEGF-A or control (PBS), and NT-pCNP production was measured. Lipopolysaccharide, TNF-α, and IL-1β significantly stimulated NT-pCNP production in a dose and time dependent manner; IL-1β resulted in the greatest NT-pCNP concentrations. The other stimulants did not result in significant NT-pCNP production. The addition of serum to the cell culture model did not alter lipopolysaccharide, lipoteichoic acid or peptidoglycan induced NT-pCNP production. These data indicate that lipopolysaccharide, TNF-α and IL-1β regulate CNP production from canine vascular endothelium and of the stimulants tested, IL-1β is the predominant inducing factor. These data provide some initial insight into the mechanisms of CNP regulation in dogs.


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