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Canine IL-21 Recombinant Protein
- Catalog Number:
- RP0256D
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Canis lupus dingo (dingo), Canis lupus familiaris (dog), Nyctereutes procyonoides (raccoon dog)
- Canine IL-21 (catalog RP0256D) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~14.9 kDa, 129 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across dog, dingo and raccoon dog. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used to study IL-21 signaling and its effects on B-cell differentiation, T-cell and NK-cell function, and antibody responses; typical experimental uses include cell-culture stimulation and dosing studies, proliferation/differentiation assays, ELISA and neutralization assays, and use as flow-cytometry and Western blot controls or for antibody generation/validation.
| Amino Acid Sequence | HKSSFQEQDL LLIRMRQLID IVDQLKNYVN DLDPESLPAP EDVKRHCERS AFSCFQKVQL KAANTGGNEQ IINVLTKQLK RKLPPTNAGR RQKHRPACPS CDSYEKAPPK EFLERLKSLI QKMIHQHLS (129) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 14.9 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Canine IL-21 (Interleukin-21) is a cytokine of the common γ chain (γc) family that plays a central role in regulating adaptive immune responses in dogs (Canis lupus familiaris). IL-21 is produced primarily by activated CD4⁺ T cells, particularly T follicular helper (Tfh) cells and Th17 cells, following antigen stimulation. It signals through the IL-21 receptor (IL-21R) in association with the common γ chain, activating downstream JAK/STAT pathways-predominantly STAT3-to modulate gene expression in target immune cells. IL-21 promotes B cell proliferation, plasma cell differentiation, immunoglobulin class switching, and antibody production, while also enhancing CD8⁺ T cell cytotoxicity and natural killer (NK) cell function. In healthy dogs, basal IL-21 expression is generally low but increases during infection, vaccination, and immune activation. Dysregulated IL-21 responses have been associated with chronic inflammatory and immune-mediated diseases such as atopic dermatitis, inflammatory bowel disease, immune-mediated hemolytic anemia, and autoimmune thyroiditis, as well as in certain lymphomas. Dogs serve as important translational animal models for human lymphoma, autoimmune disease, and allergic disorders. In veterinary and comparative medicine research, canine IL-21 is studied as a biomarker of T cell-dependent B cell help and humoral immune responses and as a potential target in immunotherapy and vaccine development.
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