Canine TNF alpha Recombinant Protein

Catalog Number:: RP0261D
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Canis lupus dingo (dingo), Canis lupus familiaris (dog), Nyctereutes procyonoides (raccoon dog)

Product Description

Canine TNF-α (catalog RP0261D) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~17.3 kDa, 157 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across several canids (dog, dingo, raccoon dog). Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable at least 3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used to study TNF-α signaling, inflammation, apoptosis and cytokine biology; typical experimental uses include cell-culture stimulation and dosing studies, cytotoxicity and apoptosis assays, ELISA and neutralization assays, receptor-binding studies, flow-cytometry and Western blot controls, and antibody generation/validation.

Specifications

Amino Acid Sequence	VKSSSRTPSD KPVAHVVANP EAEGQLQWLS RRANALLANG VELTDNQLIV PSDGLYLIYS QVLFKGQGCP STHVLLTHTI SRFAVSYQTK VNLLSAIKSP CQRETPEGTE AKPWYEPIYL GGVFQLEKGD RLSAEINLPN YLDFAESGQV YFGIIAL (157)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	17.3 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Canine Tumor Necrosis Factor Alpha (TNF-α) (also known as TNF or cachectin) is a proinflammatory cytokine that plays a central role in innate immune responses, inflammation, and host defense against pathogens in dogs (Canis lupus familiaris). TNF-α is a member of the tumor necrosis factor (TNF) superfamily, which includes related cytokines such as TNF-β (lymphotoxin-α), Fas ligand (FasL), CD40 ligand (CD40L), and TRAIL, proteins that regulate immune signaling, apoptosis, and inflammatory pathways. In dogs, TNF-α is primarily produced by activated macrophages, monocytes, dendritic cells, and T lymphocytes in response to infection, tissue injury, or inflammatory stimuli. TNF-α exerts its biological effects by binding to TNF receptors TNFR1 (p55) and TNFR2 (p75), activating intracellular signaling pathways including NF-κB, MAPK, and caspase-mediated pathways, which regulate cytokine production, immune cell activation, apoptosis, and inflammatory responses. In canine health, TNF-α is involved in immune responses to bacterial infections, viral diseases, and inflammatory conditions, including canine parvovirus infection, leishmaniasis, and chronic inflammatory disorders such as inflammatory bowel disease (IBD). Elevated TNF-α levels are also associated with sepsis, autoimmune diseases, and cancer-related inflammation, where dysregulated TNF signaling can contribute to tissue damage and disease progression. Because TNF-α is a key mediator of inflammation and immune regulation, canine TNF-α is widely studied in veterinary immunology, infectious disease research, and inflammatory disease models, contributing to improved understanding of immune responses and potential therapeutic strategies in dogs.

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