Rabbit IL-4 Recombinant Protein

Catalog Number:
RP0264U
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Oryctolagus cuniculus (rabbit)
  • Rabbit IL-4 (catalog RP0264U) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~14.2 kDa, 124 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to rabbit. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used in immunology and cell-biology research to study IL-4 signaling and Th2 immune responses, to promote B-cell proliferation and antibody class switching (including IgE), to drive differentiation of naive CD4⁺ T cells toward a Th2 phenotype, and to induce alternative (M2) macrophage activation; typical experimental uses include cell-culture stimulation and dosing studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation.
Amino Acid SequenceGRRGDIILPE VIKTLNILTE RKTPCTKLMI ADALAVPKNT TEREAVCRAA TALRQFYLHH KVSWCFKEHG ELGDLRLLRG LDRNLCSMAK LSNCPGKEAR QTTLEDFLDR LKTAMQEKYS KRQS (124)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight14.2 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Rabbit Interleukin-4 (IL-4) is a Th2-associated cytokine produced primarily by activated CD4⁺ T helper 2 (Th2) cells, mast cells, and basophils in rabbits (Oryctolagus cuniculus), where it plays a central role in regulating humoral immunity, allergic inflammation, and alternative macrophage activation. IL-4 signals through receptor complexes containing IL-4Rα paired with the common gamma chain (γc) or IL-13Rα1, activating JAK/STAT6 pathways that promote B-cell proliferation, antibody production, IgE-associated responses, and suppression of Th1-associated cytokines such as IFN-γ. In rabbit health, IL-4 is particularly relevant in parasitic infections, allergic skin conditions, and respiratory inflammation, where Th2 polarization supports antibody-mediated defense and tissue repair but may also contribute to eosinophilic infiltration and chronic inflammation if dysregulated. Rabbits are widely used as experimental models for asthma, pulmonary inflammation, atherosclerosis, and ocular inflammatory disease, and IL-4 plays a key role in airway hyperresponsiveness and mucus production in allergic airway models. As a biomarker of type 2 immune activation and a functional mediator of Th1/Th2 balance, rabbit IL-4 supports translational research into cytokine-driven allergic disease, immune polarization, and development of targeted anti-cytokine therapies relevant to human inflammatory and atopic disorders.

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1000 Westgate Drive
Suite 123
Saint Paul, MN 55114

Phone: 651-646-0089
Fax: 651-646-0095

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