Canine IFN gamma Recombinant Protein

Catalog Number:: RP0271D
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Canis lupus dingo (dingo), Canis lupus familiaris (dog), Nyctereutes procyonoides (raccoon dog), Vulpes lagopus (Arctic fox), Vulpes vulpes (red fox)

Canine IFN-γ (catalog RP0271D) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~16.7 kDa, 143 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across several canids: dog, dingo, red fox, Arctic fox, and raccoon dog. Store at -20°C (stable up to twelve months from date of receipt; working aliquots stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used in immunology and cell-biology research to study interferon-γ signaling, macrophage activation, Th1 immune responses, antigen presentation and antiviral/immunoregulatory functions; typical experimental uses include cell-culture stimulation and dosing studies, ELISA and neutralization assays, receptor-binding and signaling studies, flow-cytometry and Western blot controls, and antibody generation/validation.

Amino Acid Sequence	QAMFFKEIEN LKEYFNASNP DVSDGGSLFV DILKKWREES DKTIIQSQIV SFYLKLFDNF KDNQIIQRSM DTIKEDMLGK FLNSSTSKRE DFLKLIQIPV NDLQVQRKAI NELIKVMNDL SPRSNLRKRK RSQNLFRGRR ASK (143)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	16.7 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Canine Interferon-Gamma (IFN-γ) is a type II interferon produced primarily by activated CD4⁺ and CD8⁺ T lymphocytes and natural killer (NK) cells in dogs (Canis lupus familiaris), where it plays a central role in cell-mediated (Th1-type) immunity against intracellular pathogens. IFN-γ signals through the IFN-γ receptor complex (IFNGR1/IFNGR2), activating JAK/STAT1 pathways and inducing genes involved in macrophage activation, enhanced antigen presentation via upregulation of MHC class I and II molecules, nitric oxide production, and promotion of cytotoxic T-cell responses. In canine health, IFN-γ is critical for defense against pathogens such as Leishmania infantum, Mycobacterium spp., canine distemper virus, and certain tick-borne intracellular organisms, and its expression levels are often associated with protective Th1 immune responses and vaccine efficacy. Dysregulated or insufficient IFN-γ responses can contribute to chronic infection, immune-mediated disease, or altered inflammatory balance. In translational research, dogs serve as valuable spontaneous models for human infectious diseases (e.g., leishmaniasis), autoimmune conditions, and certain cancers, and characterization of canine IFN-γ supports studies of T-cell function, immune modulation, vaccine development, and comparative cell-mediated immunity relevant to both veterinary medicine and human health.

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