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Canine IFN gamma (Yeast-derived Recombinant Protein) - 100 micrograms

RP0271D-100
$750.00
In Stock
Data SheetAdd to Cart

Interferon gamma (IFNγ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-gamma belongs. IFN-gamma, or type II interferon, is a cytokine that is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN-gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN-gamma in the immune system stems in part from its ability to inhibit viral replication directly, but, most important, derives from its immunostimulatory and immunomodulatory effects. IFN-gamma is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.

Alternate Names - IFNG, IFG, IFI, interferon, gamma, interferon gamma

IFNγ Homology Across Species
Canis lupus familiaris (dog) IFNγ – 100%
Canis lupus dingo (dingo) IFNγ – 100%
Vulpes vulpes (red fox) IFNγ – 100%
Nyctereutes procyonoides (raccoon dog) IFNγ – 100%
Phoca vitulina (harbor seal) IFNγ – 100%
Ailurus fulgens (lesser panda) IFNγ – 96%
More - https://blast.ncbi.nlm.nih.gov

Canine IFN gamma (Yeast-derived Recombinant Protein) - 100 micrograms
Catalog No.:
RP0271D-100
Quantity:
100 ug
Source:
The Canine IFN gamma recombinant protein was produced in yeast and therefore does not have endotoxin, is naturally folded, and post-translationally modified.
MW:
The Canine IFN gamma recombinant protein has a predicted molecular weight of 16.7 kDa.
Protein Sequence:
QAMFFKEIEN LKEYFNASNP DVSDGGSLFV DILKKWREES DKTIIQSQIV SFYLKLFDNF KDNQIIQRSM DTIKEDMLGK FLNSSTSKRE DFLKLIQIPV NDLQVQRKAI NELIKVMNDL SPRSNLRKRK RSQNLFRGRR ASK (143)
Country of Origin:
USA
Applications:
The Canine IFN gamma endotoxin-free recombinant protein can be used in cell culture, as an IFN gamma ELISA Standard, and as a Western Blot Control.

32034203

TNF-α and INF-γ primed canine stem cell-derived extracellular vesicles alleviate experimental murine colitis

An JH, Li Q, Bhang DH, Song WJ, Youn HY.

Sci Rep. 2020 Feb 7;10(1):2115. doi: 10.1038/s41598-020-58909-4.

Applications: Stimulation of stem cells

Abstract

The inflammatory bowel diseases (IBD) are characterized by relapsing inflammation and immune activation diseases of the gastrointestinal tract. Extracellular vesicles, which elicit similar biological activity to the stem cell themselves, have been used experimentally to treat dextran sulfate sodium (DSS)-induced colitis in murine models though immunosuppressive potential. In this study, we investigated whether the Extracellular vesicles (EVs) obtained by stimulating inflammatory cytokine on canine adipose mesenchymal stem cells (cASC) improved anti-inflammatory and/or immunosuppressive potential of EVs, and/or their ability to alleviate inflammation in colitis. We also explored the correlation between immune cells and the inflammatory repressive effect of primed EVs. Pro-inflammatory cytokines such as TNF-α and IFN-γ increased immunosuppressive protein such as HGF, TSG-6, PGE2 and TGF-β in EVs. Moreover, the anti-inflammatory effect of EVs was improved through pretreatment with inflammatory cytokines. Importantly, EVs obtained from primed stem cells effectively induced macrophage polarization toward an anti-inflammatory M2 phenotype and suppressed activated immunity by enhancing regulatory T cells in inflamed colon in mice. Our results provide a new and effective therapy for the EVs obtained from ASC stimulated with TNF-α and IFN-γ against not only IBD, but also immune-mediated disease.


23141169

Interleukin-1β, tumour necrosis factor-α and lipopolysaccharide induce C-type natriuretic peptide from canine aortic endothelial cells.

Osterbur K, Yu DH, Declue AE.

Res Vet Sci. 2012 Nov 8. doi:pii: S0034-5288(12)00305-0. 10.1016/j.rvsc.2012.10.002.

Applications: Stimulation of canine aortic endothelial cells

Abstract
The N-terminal portion of pro C-type natriuretic peptide (NT-pCNP) has shown promise as a biomarker for sepsis in humans and dogs, however the mechanism of NT-pCNP production in dogs is unknown. Canine aortic endothelial cells were stimulated with lipopolysaccharide, lipoteichoic acid, peptidoglycan, TNF-α, IL-1β, IL-6, IL-10, IL-21, CXCL-8, IFN-γ, VEGF-A or control (PBS), and NT-pCNP production was measured. Lipopolysaccharide, TNF-α, and IL-1β significantly stimulated NT-pCNP production in a dose and time dependent manner; IL-1β resulted in the greatest NT-pCNP concentrations. The other stimulants did not result in significant NT-pCNP production. The addition of serum to the cell culture model did not alter lipopolysaccharide, lipoteichoic acid or peptidoglycan induced NT-pCNP production. These data indicate that lipopolysaccharide, TNF-α and IL-1β regulate CNP production from canine vascular endothelium and of the stimulants tested, IL-1β is the predominant inducing factor. These data provide some initial insight into the mechanisms of CNP regulation in dogs.


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