Human APRIL Recombinant Protein
- Catalog Number:
- RP0292H
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Homo sapiens (human), Pan paniscus (pygmy chimpanzee), Pan troglodytes (chimpanzee)
- Human APRIL (catalog RP0292H) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~16.4 kDa, 146 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to human and closely related pan species (pygmy chimpanzee and chimpanzee). Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study APRIL/TACI/BCMA signaling, B-cell survival, plasma-cell differentiation and antibody biology; typical experimental uses include cell-culture stimulation and dosing studies, B-cell proliferation and differentiation assays, receptor-binding and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | AVLTQKQKKQ HSVLHLVPIN ATSKDDSDVT EVMWQPALRR GRGLQAQGYG VRIQDAGVYL LYSQVLFQDV TFTMGQVVSR EGQGRQETLF RCIRSMPSHP DRAYNSCYSA GVFHLHQGDI LSVIIPRARA KLNLSPHGTF LGFVKL (146) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 16.4 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Human APRIL (A Proliferation-Inducing Ligand, also known as TNFSF13, CD256, or TALL-2) is a member of the tumor necrosis factor (TNF) superfamily that plays a key role in regulating B cell survival, proliferation, and antibody production. APRIL is primarily produced by myeloid cells such as monocytes, macrophages, dendritic cells, and neutrophils, and signals through the receptors TACI (TNFRSF13B) and BCMA (TNFRSF17), activating downstream pathways including NF-κB and MAPK that promote B cell maturation and plasma cell survival. APRIL functions closely with the related TNF family cytokine BAFF (B cell-activating factor, TNFSF13B), and together they regulate humoral immunity and long-lived antibody responses. In human health, APRIL is important for maintaining immunoglobulin production and mucosal immunity but is also implicated in pathological conditions when dysregulated, including autoimmune diseases such as systemic lupus erythematosus (SLE), rheumatoid arthritis, and multiple sclerosis, as well as B cell malignancies such as multiple myeloma and certain lymphomas. Because APRIL supports plasma cell survival and tumor growth in some cancers, it has become an important therapeutic target in immunology and oncology. Characterization of human APRIL supports research into B cell biology, antibody-mediated immunity, autoimmune disease mechanisms, and development of targeted therapies that modulate TNF superfamily signaling.
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