Feline TNF alpha Recombinant Protein

Catalog Number:: RP0294F
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Felis catus (domestic cat), Prionailurus bengalensis (leopard cat), Prionailurus viverrinus (fishing cat)

Product Description

Feline TNF-α (catalog RP0294F) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~17.2 kDa, 157 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to domestic cat, leopard cat and fishing cat. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study TNF-α signaling, inflammation, apoptosis and cytokine biology; typical experimental uses include cell-culture stimulation and dosing studies, cytotoxicity and apoptosis assays, ELISA and neutralization assays, receptor-binding studies, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Specifications

Amino Acid Sequence	LRSSSRTPSD KPVAHVVANP EAEGQLQWLS RRANALLANG VELTDNQLKV PSDGLYLIYS QVLFTGQGCP STHVLLTHTI SRFAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINLPA YLDFAESGQV YFGIIAL (157)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	17.2 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Feline Tumor Necrosis Factor Alpha (TNF-α) (also known as TNF or cachectin) is a proinflammatory cytokine that plays a central role in innate immune responses, inflammation, and host defense against pathogens in cats (Felis catus). TNF-α is a member of the tumor necrosis factor (TNF) superfamily, which includes related cytokines such as TNF-β (lymphotoxin-α), Fas ligand (FasL), CD40 ligand (CD40L), and TRAIL, molecules that regulate immune signaling, apoptosis, and inflammatory processes. In cats, TNF-α is primarily produced by activated macrophages, monocytes, dendritic cells, and T lymphocytes in response to infection, tissue injury, or inflammatory stimuli. TNF-α exerts its biological effects by binding to TNF receptors TNFR1 (p55) and TNFR2 (p75), activating intracellular signaling pathways including NF-κB, MAPK, and caspase-mediated pathways, which regulate cytokine production, immune cell activation, apoptosis, and inflammatory responses. In feline health, TNF-α is involved in immune responses to viral infections such as feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV), as well as bacterial infections, inflammatory diseases, and cancer. Dysregulated TNF-α production can contribute to chronic inflammation and tissue damage, making it an important mediator of inflammatory pathology. Because TNF-α plays a key role in immune regulation and inflammatory disease, feline TNF-α is studied in veterinary immunology, infectious disease research, and inflammatory disorder models, helping to improve understanding of immune responses and disease mechanisms in cats.

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