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Feline IL-4 Recombinant Protein
- Catalog Number:
- RP0296F
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Felis catus (domestic cat)
- Feline IL-4 (catalog RP0296F) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~12.6 kDa, 109 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to domestic cat. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used in immunology and cell-biology research to study IL-4 signaling and Th2 immune responses, to promote B-cell proliferation and antibody class switching (including IgE), to drive differentiation of naive CD4⁺ T cells toward a Th2 phenotype, and to induce alternative (M2) macrophage activation; typical experimental uses include cell-culture stimulation and dosing studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | QNFNNTLKEI IKTLNILTAR NDSCMELTVM DVLAAPKNTS DKEIFCRATT VLRQIYTHHN CSTKFLKGLD RNLSSMANRT CSVNEVKKCT LKDFLERLKA IMQKKYSKH (109) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 12.6 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Feline Interleukin-4 (IL-4) is a Th2-associated cytokine produced primarily by activated CD4⁺ T helper 2 (Th2) cells, mast cells, and basophils in cats (Felis catus), where it plays a central role in regulating humoral immunity, allergic inflammation, and alternative macrophage activation. IL-4 signals through receptor complexes containing IL-4Rα paired with the common gamma chain (γc) or IL-13Rα1, activating JAK/STAT6 pathways that promote B-cell proliferation, antibody production, IgE-associated responses, and suppression of certain pro-inflammatory Th1 cytokines. In feline health, IL-4 is particularly relevant in allergic and immune-mediated conditions such as feline asthma, atopic dermatitis, eosinophilic granuloma complex, and food hypersensitivity, where Th2 polarization drives eosinophilic inflammation and airway or skin pathology. IL-4 may also influence immune balance during chronic viral infections such as feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) by shaping Th1/Th2 dynamics and potentially affecting viral persistence. As a biomarker of type 2 immune activation, feline IL-4 is important in studies of allergic disease pathogenesis, vaccine responses, and immune regulation. In translational research, cats serve as valuable spontaneous models for human asthma and allergic airway disease, and characterization of feline IL-4 supports comparative investigations into Th2-mediated inflammation and development of targeted anti-cytokine therapies relevant to both veterinary and human medicine.
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