Bovine IFN beta Recombinant Protein

Catalog Number:: RP0298B
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Bos taurus (cattle)

Product Description

Bovine IFN-β (catalog RP0298B) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~19.4 kDa, 164 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to cattle. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study interferon-β signaling and antiviral responses, induction of interferon-stimulated genes and antiviral states, and for antiviral/cytopathic-effect assays; typical experimental uses include cell-culture stimulation and dosing studies, ELISA and neutralization assays, receptor-binding and signaling studies, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Specifications

Amino Acid Sequence	SYSLLRFQQR QSLKECQKLL GQLPSTPQHC LEARMDFQMP EEMKQEQQFQ KEDAILVMYE VLQHIFGILT RDFSSTGWSE TIIEDLLEEL YGQMNRLQPI QKEIMQKQNT TAGDTIVPHL GKYYFNLMQY LESKEYDRCA WTVVQVQILT NVSFLMRLTG YVRD (164)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	19.4 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Bovine Interferon-Beta (IFN-β) is a type I interferon produced primarily by virus-infected epithelial cells, fibroblasts, macrophages, and dendritic cells in cattle (Bos taurus), where it serves as a key early mediator of innate antiviral immunity. Following recognition of viral nucleic acids by pattern recognition receptors such as RIG-I-like receptors and the cGAS-STING pathway, bovine IFN-β is rapidly induced and signals through the type I interferon receptor complex (IFNAR1/IFNAR2), activating JAK/STAT pathways and driving expression of interferon-stimulated genes (ISGs) that inhibit viral replication, enhance antigen presentation, and promote natural killer (NK) cell and adaptive immune activation. IFN-β plays an important role in host defense against economically significant bovine viral pathogens including bovine viral diarrhea virus (BVDV), bovine herpesvirus-1 (IBR), bovine respiratory syncytial virus (BRSV), foot-and-mouth disease virus (FMDV), and other agents involved in the bovine respiratory disease (BRD) complex. Many of these viruses possess immune evasion strategies that suppress or delay IFN-β production, contributing to viral persistence and disease severity. As both a biomarker and functional mediator of antiviral responses, bovine IFN-β is central to vaccine evaluation, antiviral research, and studies of host-virus interactions in cattle, while also supporting comparative research into type I interferon biology in large-animal models relevant to human infectious disease.

Choose a size

Please choose a size to see price

RELATED MEDIA