Swine IL-4 Recombinant Protein

Catalog Number:: RP0300S
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Phacochoerus africanus (Common warthog), Sus scrofa (pig)

Product Description

Swine IL-4 (catalog RP0300S) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~12.5 kDa, 109 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to pig and the common warthog. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used in immunology and cell-biology research to study IL-4 signaling and Th2 immune responses, to promote B-cell proliferation and antibody class switching (including IgE), to drive differentiation of naive CD4⁺ T cells toward a Th2 phenotype, and to induce alternative (M2) macrophage activation; typical experimental uses include cell-culture stimulation and dosing studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Specifications

Amino Acid Sequence	HKCDITLQEI IKTLNILTAR KNSCMELPVT DVFAAPENTT EKETFCRAST VLRHIYRHHT CMKSLLSGLD RNLSSMANMT CSVHEAKKST LKDFLERLKT IMKEKYSKC (109)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	12.5 kda
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Swine Interleukin-4 (IL-4) is a Th2-associated cytokine produced primarily by activated CD4⁺ T helper 2 (Th2) cells, mast cells, and basophils in pigs (Sus scrofa domesticus), where it plays a central role in regulating humoral immunity, alternative macrophage activation, and modulation of inflammatory responses. IL-4 signals through receptor complexes containing IL-4Rα paired with the common gamma chain (γc) or IL-13Rα1, activating JAK/STAT6 pathways that promote B-cell proliferation, antibody production, and suppression of Th1-associated cytokines such as IFN-γ. In swine health, IL-4 is particularly important in responses to gastrointestinal and respiratory parasitic infections, where Th2 polarization supports parasite clearance and mucosal repair. IL-4 also influences immune balance during viral infections such as porcine reproductive and respiratory syndrome virus (PRRSV) and swine influenza, where Th1/Th2 dynamics can affect viral persistence, vaccine responsiveness, and disease outcome. While IL-4-mediated responses are protective in supporting humoral immunity and limiting excessive inflammation, excessive or prolonged Th2 skewing may impair effective cell-mediated responses against intracellular pathogens. As a biomarker of type 2 immune activation, swine IL-4 is relevant in vaccine development, host-parasite interaction studies, and immune resilience research. In translational research, pigs serve as valuable large-animal models due to physiological similarities to humans, and characterization of swine IL-4 supports comparative investigations into Th2-mediated immunity and cytokine-targeted therapeutic strategies.

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