Swine IL-10 Recombinant Protein

Catalog Number:: RP0301S
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Sus scrofa (pig)

Product Description

Swine IL-10 (catalog RP0301S) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~18.1 kDa, 157 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to pig. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-10 signaling and anti-inflammatory/regulatory functions (macrophage/monocyte regulation, Treg biology and suppression of pro-inflammatory cytokines); typical experimental uses include cell-culture stimulation and dosing studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, cytokine signaling assays, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Specifications

Amino Acid Sequence	SIKSENSCIH FPTSLPHMLR ELRAAFGPVK SFFQTKDQMG DLLLTGSLLE DFKGYLGCQA LSEMIQFYLE DVMPKAESDG EDIKEHVNSL GEKLKTLRLR LRRCHQFLPC ENKSKAVEEV KSAFSKLQER GVYKAMGEFD IFINYIEAYM TMKMRKN (157)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	18.1 kda
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Swine Interleukin-10 (IL-10) is a key anti-inflammatory cytokine produced by regulatory T cells (Tregs), Th2 cells, macrophages, dendritic cells, and certain B-cell populations in pigs (Sus scrofa domesticus), where it plays a central role in limiting excessive immune activation and maintaining immune homeostasis. IL-10 signals through the IL-10 receptor complex (IL-10R1/IL-10R2), activating JAK/STAT3 pathways that suppress pro-inflammatory cytokine production (including IL-1β, TNF-α, IL-6, and IFN-γ), reduce antigen presentation, and downregulate Th1-type cell-mediated responses. In swine health, IL-10 is critically involved in modulating immune responses during economically significant infections such as porcine reproductive and respiratory syndrome virus (PRRSV), African swine fever virus (ASFV), swine influenza virus, enteric infections, and bacterial pneumonia, where elevated IL-10 can limit tissue damage but may also contribute to immune suppression and viral persistence. The balance between IL-10 and pro-inflammatory cytokines influences disease severity, vaccine responsiveness, and growth performance. As a biomarker of immune regulation, swine IL-10 is widely used in studies of vaccine efficacy, immunopathogenesis, and herd health management. In translational research, pigs serve as valuable large-animal models due to physiological similarities to humans, and characterization of swine IL-10 supports investigations into immune tolerance, chronic infection dynamics, cytokine network regulation, and development of immunomodulatory strategies relevant to both veterinary and human medicine.

Choose a size

Please choose a size to see price

RELATED MEDIA