Bovine IL-18 Recombinant Protein

Catalog Number:: RP0304B
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Bos indicus (zebu), Bos taurus (cattle)

Bovine IL-18 (catalog RP0304B) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~18.1 kDa, 157 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to cattle and zebu (note: material contains a Lys121 to Glu substitution). Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-18 signaling, induction of IFN-γ and Th1 responses, NK- and T-cell activation, and inflammasome/ inflammatory biology; typical experimental uses include cell-culture stimulation and dosing studies, IFN-γ induction and cytokine assays, ELISA and neutralization assays, receptor-binding and signaling studies, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Amino Acid Sequence	HFGKLEPKLS IIRNLNDQVL FINQGNQPVF EDMPDSDCSD NAPQTIFIIY MYKDSLTRGL AVTISVQCKK MSTLSCENKI VSFKEMNPPD NIDNEESDII FFQRSVPGHD DKIQFESSLY EGYFLACKKE NDLFKLILKK QDDNRDKSVM FTVQNQN (157)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	18.1 kda
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Bovine IL-18 (Interleukin-18) is a pro-inflammatory cytokine belonging to the IL-1 family that plays a central role in innate and adaptive immune responses in cattle (Bos taurus). IL-18 is produced primarily by monocytes, macrophages, dendritic cells, and epithelial cells as an inactive precursor that requires cleavage by caspase-1 following inflammasome activation to become biologically active. Once activated, IL-18 stimulates production of interferon-γ (IFN-γ) by T cells and natural killer (NK) cells, thereby promoting Th1-type immune responses and enhancing cell-mediated immunity against intracellular pathogens. In healthy cattle, basal IL-18 expression is generally low, but levels increase during bacterial and viral infections, mastitis, respiratory disease, and inflammatory stimulation. IL-18 has been implicated in immune responses to pathogens such as Mycobacterium bovis, bovine viral diarrhea virus (BVDV), and agents of bovine respiratory disease complex. In veterinary and agricultural research, bovine IL-18 serves as a biomarker of inflammasome activation and Th1-mediated immune activation, providing insight into host-pathogen interactions, vaccine responses, and inflammatory disease mechanisms in cattle.

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