Turkey IL-8 Recombinant Protein

Catalog Number:
RP0306T
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Meleagris gallopavo (turkey)
  • Turkey IL-8 (catalog RP0306T) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~9.6 kDa, 88 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to turkey. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study chemokine signaling and leukocyte chemoattraction/migration; typical experimental uses include chemotaxis and cell-migration assays, cell-culture stimulation and dosing studies, receptor-binding and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceAALSQGRTLV KMGNELRCQC ISTHSKFIHP KSIQDVKLTP SGPHCKNVEI IATLKDSREV CLDPTAPWVQ LIVKALMAKA QLNSDAPL (88)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight9.6 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Turkey (Meleagris gallopavo) Interleukin-8 (IL-8), functionally analogous to avian CXCLi2 (and closely related to CXCLi1), is a key pro-inflammatory chemokine that regulates heterophil chemotaxis and activation in response to infection and tissue injury. Produced by macrophages, epithelial cells, and other immune cells following bacterial, viral, or parasitic challenge, turkey IL-8 signals through CXCR1- and CXCR2-like receptors to promote recruitment of heterophils—the avian equivalent of neutrophils—to affected tissues. IL-8 is rapidly upregulated during economically significant turkey diseases such as avian influenza, hemorrhagic enteritis virus, Salmonella infection, Eimeria (coccidiosis), Clostridium infections, and Mycoplasma gallisepticum, where it contributes to early innate immune defense but may also drive inflammatory tissue damage if dysregulated. Elevated IL-8 expression in respiratory and gastrointestinal tissues serves as an important biomarker of mucosal immune activation and disease severity in commercial flocks. In research settings, turkeys serve as relevant models for avian respiratory and enteric disease pathogenesis, vaccine evaluation, and heterophil-driven inflammatory responses, and characterization of IL-8 enhances understanding of chemokine-mediated innate immunity and inflammatory regulation in poultry health and comparative immunology.

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