Equine IL-8 Recombinant Protein

Catalog Number:: RP0312E
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Equus caballus (horse), Equus przewalskii (Przewalski's horse)

Equine IL-8 (catalog RP0312E) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~8.9 kDa, 79 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to horse and Przewalski's horse. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study chemokine signaling and leukocyte chemoattraction/migration; typical experimental uses include chemotaxis and cell-migration assays, cell-culture stimulation and dosing studies, receptor-binding and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Amino Acid Sequence	AVVSRITAEL RCQCIKTHSK PFNPKLIKEM RVIESGPHCE NSEIIVKLVN GAEVCLNPHT KWVQIIVQAF LKRAEGQNP (79)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	8.9 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Equine Interleukin-8 (IL-8), also known as CXCL8, is a potent pro-inflammatory chemokine that plays a central role in innate immune responses in horses (Equus caballus) by mediating neutrophil chemotaxis, activation, and migration to sites of infection or tissue injury. Produced by macrophages, respiratory and intestinal epithelial cells, endothelial cells, and synoviocytes in response to microbial stimuli and inflammatory cytokines, equine IL-8 signals primarily through CXCR1 and CXCR2 receptors to promote rapid neutrophil recruitment and amplification of acute inflammation. Elevated IL-8 expression is associated with common equine conditions such as recurrent airway obstruction (equine asthma), bacterial pneumonia, strangles (Streptococcus equi infection), colitis, endotoxemia, osteoarthritis, and laminitis, where excessive neutrophilic inflammation can contribute to tissue damage and disease severity. As a biomarker, IL-8 is valuable for assessing inflammatory status, monitoring respiratory and joint disease progression, and evaluating treatment responses. In research settings, horses serve as important large-animal models for human asthma, osteoarthritis, sepsis, and exercise-induced inflammation, and characterization of equine IL-8 enhances understanding of neutrophil-driven pathology, mucosal immune responses, and chemokine-targeted therapeutic strategies relevant to both veterinary and human medicine.

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