Guinea Pig TNF alpha Recombinant Protein

Catalog Number:: RP0345GP
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Cavia porcellus (domestic guinea pig)

Product Description

Guinea Pig TNF-α (catalog RP0345GP) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~17.0 kDa, 154 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to guinea pig. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study TNF-α signaling, inflammation, apoptosis and cytokine biology; typical experimental uses include cell-culture stimulation and dosing studies, cytotoxicity and apoptosis assays, ELISA and neutralization assays, receptor-binding studies, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Specifications

Amino Acid Sequence	SASQNDNDKP VAHVVANQQA EEELQWLSKR ANALLANGMG LSDNQLVVPS DGLYLIYSQV LFKGQGCPSY LLLTHTVSRL AVSYPEKVNL LSAIKSPCQK ETPEGAERKP WYEPIYLGGV FQLQKGDRLS AEVNLPQYLD FADSGQIYFG VIAL (154)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	17.0 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Guinea Pig Tumor Necrosis Factor Alpha (TNF-α) (also known as TNF or cachectin) is a proinflammatory cytokine that plays a central role in innate immune responses, inflammation, and host defense against pathogens in guinea pigs (Cavia porcellus). TNF-α is a member of the tumor necrosis factor (TNF) superfamily, which includes related cytokines such as TNF-β (lymphotoxin-α), Fas ligand (FasL), CD40 ligand (CD40L), and TRAIL, proteins that regulate immune signaling, apoptosis, and inflammatory pathways. In guinea pigs, TNF-α is primarily produced by activated macrophages, monocytes, dendritic cells, and T lymphocytes in response to infection, endotoxin exposure, or tissue injury. TNF-α exerts its biological effects by binding to TNF receptors TNFR1 (p55) and TNFR2 (p75), activating intracellular signaling pathways including NF-κB, MAPK, and caspase-mediated pathways, which regulate cytokine production, immune cell activation, apoptosis, and inflammatory responses. In guinea pigs, TNF-α is particularly important in immune responses to intracellular bacterial infections such as Mycobacterium tuberculosis, for which guinea pigs serve as a well-established animal model of human tuberculosis due to their similar disease pathology and granuloma formation. TNF-α contributes to macrophage activation, granuloma maintenance, and containment of mycobacterial infection, although excessive TNF signaling can also contribute to tissue inflammation and pathology. Because guinea pigs are widely used in infectious disease, immunology, and tuberculosis research, characterization of guinea pig TNF-α supports studies of cytokine regulation, host-pathogen interactions, and inflammatory responses relevant to both veterinary and human health.

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