Human IL-8 Recombinant Protein

Catalog Number:: RP0357H
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Gorilla gorilla gorilla (western lowland gorilla), Homo sapiens (human), Pan paniscus (pygmy chimpanzee), Pan troglodytes (chimpanzee)

Human IL-8 (catalog RP0357H) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~9.1 kDa, 79 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to human, western lowland gorilla, pygmy chimpanzee and chimpanzee. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study chemokine signaling and leukocyte (particularly neutrophil) chemoattraction/migration; typical experimental uses include chemotaxis and cell-migration assays, cell-culture stimulation and dosing studies, receptor-binding and signaling assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Amino Acid Sequence	EGAVLPRSAK ELRCQCIKTY SKPFHPKFIK ELRVIESGPH CANTEIIVKL SDGRELCLDP KENWVQRVVE KFLKRAENS (79)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	9.1 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Human Interleukin-8 (IL-8), also known as CXCL8, is a potent pro-inflammatory chemokine that plays a central role in innate immunity by mediating neutrophil chemotaxis, activation, degranulation, and respiratory burst at sites of infection or tissue injury. Produced by macrophages, monocytes, epithelial cells, endothelial cells, and fibroblasts in response to microbial products and inflammatory cytokines such as IL-1β and TNF-α, IL-8 signals primarily through the G protein-coupled receptors CXCR1 and CXCR2 to drive rapid neutrophil recruitment and amplification of inflammatory cascades. Elevated IL-8 levels are associated with numerous acute and chronic conditions, including sepsis, acute respiratory distress syndrome (ARDS), chronic obstructive pulmonary disease (COPD), asthma, inflammatory bowel disease, rheumatoid arthritis, psoriasis, and multiple cancers where IL-8 contributes to tumor angiogenesis, metastasis, and immune modulation. Dysregulated IL-8 signaling is implicated in cytokine storm syndromes and severe viral infections, including influenza and COVID-19. As both a biomarker and therapeutic target, human IL-8 is central to research on neutrophil-driven inflammation, cancer progression, and immune-mediated tissue injury, with CXCR1/CXCR2 antagonists and pathway inhibitors under investigation for inflammatory and oncologic indications.

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