Ovine IL-6 Recombinant Protein

Catalog Number:: RP0367V
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Ovis aries (sheep)

Product Description

Ovine IL-6 (catalog RP0367V) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~20.4 kDa, 179 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to sheep. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-6 signaling, acute-phase and inflammatory responses, and B-cell differentiation; typical experimental uses include cell-culture stimulation and dosing studies, proliferation/differentiation assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Specifications

Amino Acid Sequence	GPLGEDFKND TTPSRLLLTT PEKTEALIKH IVDKISAIRK EICEKNDECE NSKETLAENK LKLPKMEEKD GCFQSGFNQA ICLIKTTAGL LEYQIYLDFL QNEFEGNQET VMELQSSIRT LIQILKEKIA GLITTPATHT DMLEKMQSSN EWVKNAKVII ILRSLENFLQ FSLRAIRMK (179)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	20.4 kda
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Ovine Interleukin-6 (IL-6) is a multifunctional pro-inflammatory cytokine and a central member of the IL-6 cytokine family-including IL-6, IL-11, IL-27, IL-31, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1), and cardiotrophin-like cytokine factor 1 (CLCF1)-which signal through receptor complexes containing the shared gp130 subunit to activate JAK/STAT and MAPK pathways. In sheep (Ovis aries), IL-6 plays a critical role in regulating acute-phase protein production (such as haptoglobin and serum amyloid A), leukocyte recruitment, B- and T-cell differentiation, fever induction, and systemic inflammatory responses during infection, tissue injury, and metabolic stress. Elevated IL-6 expression is associated with economically significant ovine diseases including mastitis, pneumonia, foot rot, caseous lymphadenitis, parasitic infestations, and viral infections such as ovine progressive pneumonia (maedi-visna), where it contributes to inflammation and disease severity. IL-6 also plays an important role in reproductive physiology and periparturient immune modulation. As a biomarker of immune activation, ovine IL-6 is valuable for herd health monitoring, vaccine evaluation, and assessment of inflammatory status. In veterinary and translational research, characterization of ovine IL-6 and related family members supports studies of host-pathogen interactions, small ruminant immunology, reproductive immunology, and development of immune-based strategies to improve animal health, productivity, and welfare.

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