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Bovine APRIL Recombinant Protein
- Catalog Number:
- RP0376B
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Bison bison bison (American buffalo), Bos indicus (zebu), Bos javanicus (banteng), Bos mutus (wild yak), Bos taurus (cattle)
- Bovine APRIL (catalog RP0376B) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~16.5 kDa, 146 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across several bovine species (cattle, American buffalo, zebu, banteng, and wild yak). Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study APRIL/TACI/BCMA signaling, B-cell survival, plasma-cell differentiation and antibody biology; typical experimental uses include cell-culture stimulation and dosing studies, B-cell proliferation and differentiation assays, receptor-binding and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | AVLTRKHKKK RSVLHLVPIN ITSKEDSDVT EVMWQPALQR GRGLEAQGYV VRVWDAGVYL LYSQVLFHDE TFTMGQMVSR EGQGRQETLF RCIQSMPSNP DWAYNSCYSA GVFHLHQGDI LSVVIPRARA KLSLSPHGTF LGLVKL (146) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 16.5 kda |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Bovine APRIL (A Proliferation-Inducing Ligand), also known as TNFSF13, is a member of the tumor necrosis factor (TNF) superfamily that plays a central role in B cell survival, plasma cell maintenance, and regulation of humoral immunity in cattle (Bos taurus). APRIL is produced primarily by monocytes, macrophages, dendritic cells, and certain epithelial and stromal cells, and it signals through the receptors TACI (TNFRSF13B) and BCMA (TNFRSF17) to promote B cell maturation, immunoglobulin class switching, and the persistence of long-lived antibody-secreting plasma cells. In bovine immune responses, APRIL contributes to mucosal immunity and IgA production, particularly in the respiratory and gastrointestinal tracts and the mammary gland. Dysregulated APRIL expression may be associated with chronic infection, persistent inflammation, and B cell-related diseases such as bovine leukemia virus (BLV)-associated lymphoproliferative disorders. In veterinary and translational research, bovine APRIL is of interest in studies of vaccine responses, mastitis immunity, mucosal antibody production, and comparative B cell biology, providing insight into antibody-mediated immune regulation and potential strategies to enhance protective immunity in cattle.
5 ug
Price: $170.00
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