Mouse FGF basic Recombinant Protein

Catalog Number:
RP0402M
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Acomys russatus (golden spiny mouse), Mastomys coucha (southern multimammate mouse), Meriones unguiculatus (Mongolian gerbil), Mus musculus (house mouse), Mus pahari (shrew mouse)
  • Mouse FGF basic (catalog RP0402M) is a yeast-derived growth factor supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~16.2 kDa, 144 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology across several rodent species (house mouse, shrew mouse, golden spiny mouse, Mongolian gerbil, and southern multimammate mouse). Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study FGF/FGFR signaling, cell proliferation and survival, stem-cell maintenance, angiogenesis and tissue-repair biology; typical experimental uses include cell-culture stimulation and dosing studies, proliferation and differentiation assays, receptor-binding and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceALPEDGGAAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHVK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTEEC FFFERLESNN YNTYRSRKYS SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS (144)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight16.2 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Affinity TagNone
aminoAcids144
Carrier ProteinNone
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Mouse Basic Fibroblast Growth Factor (FGF2, also known as bFGF) is a heparin-binding growth factor belonging to the fibroblast growth factor (FGF) family, which includes more than 20 related proteins that regulate cell proliferation, differentiation, angiogenesis, and tissue repair. In mice (Mus musculus), FGF2 is produced by fibroblasts, endothelial cells, macrophages, neurons, and other stromal and epithelial cells, where it plays key roles in development, wound healing, and vascular growth. FGF2 binds to fibroblast growth factor receptors (FGFR1–FGFR4) in the presence of heparan sulfate proteoglycans, activating intracellular signaling pathways such as MAPK/ERK, PI3K–AKT, and PLCγ, which promote cell proliferation, survival, migration, and angiogenesis. In murine physiology, FGF2 is particularly important in neurodevelopment, skeletal development, and tissue regeneration, including skin and vascular repair. Mice are widely used as animal models for studying growth factor signaling and regenerative biology, and FGF2 has been extensively investigated in mouse models of wound healing and angiogenesis, where FGF2 signaling regulates endothelial cell proliferation and new blood vessel formation, helping researchers understand mechanisms of tissue repair, cancer growth, and therapeutic angiogenesis relevant to human medicine.
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Suite 123
Saint Paul, MN 55114

Phone: 651-646-0089
Fax: 651-646-0095

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