Mouse BAFF Recombinant Protein

Catalog Number:
RP0405M
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Mus caroli (Ryukyu mouse), Mus musculus (house mouse)
  • Mouse BAFF (catalog RP0405M) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~20.6 kDa, 183 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to house mouse and Ryukyu mouse. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study BAFF/BAFF-R signaling, B-cell survival and maturation, and antibody/immune regulation; typical experimental uses include cell-culture stimulation and dosing studies, B-cell proliferation and differentiation assays, receptor-binding and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceAFQGPEETEQ DVDLSAPPAP CLPGCRHSQH DDNGMNLRNI IQDCLQLIAD SDTPTIRKGT YTFVPWLLSF KRGNALEEKE NKIVVRQTGY FFIYSQVLYT DPIFAMGHVI QRKKVHVFGD ELSLVTLFRC IQNMPKTLPN NSCYSAGIAR LEEGDEIQLA IPRENAQISR NGDDTFFGAL KLL (183)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight20.6 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Affinity TagNone
aminoAcids183
Carrier ProteinNone
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Mouse BAFF (B cell–Activating Factor), also known as BLyS or TNFSF13B, is a member of the tumor necrosis factor (TNF) superfamily and is a key regulator of B cell development, survival, and humoral immunity in mice (Mus musculus). BAFF is produced primarily by monocytes, macrophages, dendritic cells, neutrophils, and stromal cells, and it signals through the receptors BAFF-R (TNFRSF13C), TACI (TNFRSF13B), and BCMA (TNFRSF17) to promote peripheral B cell maturation, immunoglobulin class switching, and maintenance of long-lived plasma cells. In mice, BAFF is essential for transitional B cell survival and follicular B cell homeostasis; BAFF-deficient mice exhibit reduced peripheral B cell numbers, while BAFF overexpression leads to B cell hyperplasia and autoimmunity. Dysregulated BAFF signaling has been implicated in mouse models of systemic lupus erythematosus (SLE), rheumatoid arthritis, multiple sclerosis, and other antibody-mediated autoimmune diseases. In biomedical research, mouse BAFF is widely studied in models of autoimmunity, chronic inflammation, transplant rejection, vaccine responses, and B cell malignancies, where it serves as a critical mediator of B cell–driven immune regulation and a therapeutic target for modulating pathological antibody production.
  • Lupus
  • Sjogren's Syndrome
  • TNF Superfamily
  • immunology
  • Vaccine Development

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Saint Paul, MN 55114

Phone: 651-646-0089
Fax: 651-646-0095

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