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Swine APRIL Recombinant Protein
- Catalog Number:
- RP0415S
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Sus scrofa (pig)
- Swine APRIL (catalog RP0415S) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~16.5 kDa, 146 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to pig. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study APRIL/TNFSF13 signaling and B-cell biology (including survival, proliferation, and antibody production); typical experimental uses include cell-culture stimulation and dosing studies, proliferation assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | AVLTRKQKKK RSVLHLVPTN ITSKEDSDVT ELMWQPALKR GRGLEAQGYF VRVWDAGVYL LYSQVLFHDV TFTMGQVVSR EGQGRQETLF RCIRSMPSNP DWAYNSCYSA GVFHLHQGDI LSVVIPRARA KLSLSPHGTF LGVVKL (146) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 16.5 kda |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Swine APRIL (A Proliferation-Inducing Ligand), also known as TNFSF13, is a member of the tumor necrosis factor (TNF) superfamily that plays an important role in B cell survival, plasma cell maintenance, and regulation of humoral immunity in pigs (Sus scrofa). APRIL is produced primarily by monocytes, macrophages, dendritic cells, and certain epithelial and stromal cells, and it signals through the receptors TACI (TNFRSF13B) and BCMA (TNFRSF17) to promote B cell maturation, immunoglobulin class switching, and the persistence of long-lived antibody-secreting plasma cells. In swine, APRIL contributes to mucosal immunity, including IgA production in the respiratory and gastrointestinal tracts, which is particularly relevant for defense against enteric and respiratory pathogens. Dysregulated APRIL expression may be associated with chronic infection, persistent inflammation, or B cell dysregulation in porcine disease models. In veterinary and translational research, swine APRIL is of interest in studies of vaccine-induced antibody responses, mucosal immunity, porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza, and other infectious disease models, as well as in comparative immunology research due to physiological similarities between pigs and humans. Characterizing APRIL signaling in swine provides valuable insight into antibody-mediated immunity and strategies to enhance protective immune responses in both agricultural and biomedical settings.
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